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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus anthracis Thiamine-phosphate synthase (thiE) CSB-YP501470BQG
CSB-EP501470BQG
CSB-BP501470BQG
CSB-MP501470BQG
CSB-EP501470BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0435 protein BAMEG_0475 (BAMEG_0475) CSB-YP501471BQG
CSB-EP501471BQG
CSB-BP501471BQG
CSB-MP501471BQG
CSB-EP501471BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Metallothiol transferase fosB 2 (fosB2) CSB-YP501472BQG
CSB-EP501472BQG
CSB-BP501472BQG
CSB-MP501472BQG
CSB-EP501472BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 50S ribosomal protein L32 (rpmF) CSB-YP501473BQG
CSB-EP501473BQG
CSB-BP501473BQG
CSB-MP501473BQG
CSB-EP501473BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Uncharacterized N-acetyltransferase BAMEG_0567 (BAMEG_0567) CSB-YP501474BQG
CSB-EP501474BQG
CSB-BP501474BQG
CSB-MP501474BQG
CSB-EP501474BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0747 protein BAMEG_0569 (BAMEG_0569), partial CSB-YP501475BQG
CSB-EP501475BQG
CSB-BP501475BQG
CSB-MP501475BQG
CSB-EP501475BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP501476BQG
CSB-EP501476BQG
CSB-BP501476BQG
CSB-MP501476BQG
CSB-EP501476BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Deoxyribose-phosphate aldolase (deoC) CSB-YP501477BQG
CSB-EP501477BQG
CSB-BP501477BQG
CSB-MP501477BQG
CSB-EP501477BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0176 protein BAMEG_2712 (BAMEG_2712) CSB-YP501478BQG
CSB-EP501478BQG
CSB-BP501478BQG
CSB-MP501478BQG
CSB-EP501478BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP501479BQG
CSB-EP501479BQG
CSB-BP501479BQG
CSB-MP501479BQG
CSB-EP501479BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Dihydroxy-acid dehydratase (ilvD), partial CSB-YP501480BQG
CSB-EP501480BQG
CSB-BP501480BQG
CSB-MP501480BQG
CSB-EP501480BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Aspartate--ammonia ligase (asnA) CSB-YP501481BQG
CSB-EP501481BQG
CSB-BP501481BQG
CSB-MP501481BQG
CSB-EP501481BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP501482BQG
CSB-EP501482BQG
CSB-BP501482BQG
CSB-MP501482BQG
CSB-EP501482BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP501483BQG
CSB-EP501483BQG
CSB-BP501483BQG
CSB-MP501483BQG
CSB-EP501483BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP501484BQG
CSB-EP501484BQG
CSB-BP501484BQG
CSB-MP501484BQG
CSB-EP501484BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Quinolinate synthase A (nadA) CSB-YP501485BQG
CSB-EP501485BQG
CSB-BP501485BQG
CSB-MP501485BQG
CSB-EP501485BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 50S ribosomal protein L27 (rpmA) CSB-YP501486BQG
CSB-EP501486BQG
CSB-BP501486BQG
CSB-MP501486BQG
CSB-EP501486BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 50S ribosomal protein L21 (rplU) CSB-YP501487BQG
CSB-EP501487BQG
CSB-BP501487BQG
CSB-MP501487BQG
CSB-EP501487BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Probable septum site-determining protein MinC (minC) CSB-YP501488BQG
CSB-EP501488BQG
CSB-BP501488BQG
CSB-MP501488BQG
CSB-EP501488BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0758 protein BAMEG_4721 (BAMEG_4721) CSB-YP501489BQG
CSB-EP501489BQG
CSB-BP501489BQG
CSB-MP501489BQG
CSB-EP501489BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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