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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Bacillus anthracis SsrA-binding protein (smpB) CSB-YP503270BQF
CSB-EP503270BQF
CSB-BP503270BQF
CSB-MP503270BQF
CSB-EP503270BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Enolase (eno) CSB-YP503271BQF
CSB-EP503271BQF
CSB-BP503271BQF
CSB-MP503271BQF
CSB-EP503271BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoglycerate kinase (pgk) CSB-YP503272BQF
CSB-EP503272BQF
CSB-BP503272BQF
CSB-MP503272BQF
CSB-EP503272BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Putative sporulation transcription regulator WhiA (whiA) CSB-YP503273BQF
CSB-EP503273BQF
CSB-BP503273BQF
CSB-MP503273BQF
CSB-EP503273BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0042 nucleotide-binding protein BAA_5414 (BAA_5414) CSB-YP503274BQF
CSB-EP503274BQF
CSB-BP503274BQF
CSB-MP503274BQF
CSB-EP503274BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Pyrophosphatase ppaX (ppaX) CSB-YP503275BQF
CSB-EP503275BQF
CSB-BP503275BQF
CSB-MP503275BQF
CSB-EP503275BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP503276BQF1
CSB-EP503276BQF1
CSB-BP503276BQF1
CSB-MP503276BQF1
CSB-EP503276BQF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis HPr kinase/phosphorylase (hprK) CSB-YP503277BQF
CSB-EP503277BQF
CSB-BP503277BQF
CSB-MP503277BQF
CSB-EP503277BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis D-ribose pyranase (rbsD) CSB-YP503278BQF
CSB-EP503278BQF
CSB-BP503278BQF
CSB-MP503278BQF
CSB-EP503278BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Thiazole synthase (thiG) CSB-YP503279BQF
CSB-EP503279BQF
CSB-BP503279BQF
CSB-MP503279BQF
CSB-EP503279BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Potassium-transporting ATPase A chain (kdpA), partial CSB-YP503280BQF
CSB-EP503280BQF
CSB-BP503280BQF
CSB-MP503280BQF
CSB-EP503280BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Potassium-transporting ATPase C chain (kdpC), partial CSB-YP503281BQF1
CSB-EP503281BQF1
CSB-BP503281BQF1
CSB-MP503281BQF1
CSB-EP503281BQF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Pyrrolidone-carboxylate peptidase (pcp) CSB-YP503282BQF
CSB-EP503282BQF
CSB-BP503282BQF
CSB-MP503282BQF
CSB-EP503282BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0271 protein BAA_3146 (BAA_3146) CSB-YP503283BQF
CSB-EP503283BQF
CSB-BP503283BQF
CSB-MP503283BQF
CSB-EP503283BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0753 protein BAA_3232 (BAA_3232), partial CSB-YP503284BQF
CSB-EP503284BQF
CSB-BP503284BQF
CSB-MP503284BQF
CSB-EP503284BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Protein ArsC (arsC) CSB-YP503285BQF
CSB-EP503285BQF
CSB-BP503285BQF
CSB-MP503285BQF
CSB-EP503285BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphomethylpyrimidine synthase (thiC), partial CSB-YP503286BQF
CSB-EP503286BQF
CSB-BP503286BQF
CSB-MP503286BQF
CSB-EP503286BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Transcriptional regulator lytR (lytR), partial CSB-YP503287BQF
CSB-EP503287BQF
CSB-BP503287BQF
CSB-MP503287BQF
CSB-EP503287BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP503288BQF
CSB-EP503288BQF
CSB-BP503288BQF
CSB-MP503288BQF
CSB-EP503288BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP503289BQF1
CSB-EP503289BQF1
CSB-BP503289BQF1
CSB-MP503289BQF1
CSB-EP503289BQF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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