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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sulfolobus islandicus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP503210FPG
CSB-EP503210FPG
CSB-BP503210FPG
CSB-MP503210FPG
CSB-EP503210FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable deoxyhypusine synthase (dys) CSB-YP503211FPG
CSB-EP503211FPG
CSB-BP503211FPG
CSB-MP503211FPG
CSB-EP503211FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Type 2 DNA topoisomerase 6 subunit B (top6B), partial CSB-YP503212FPG
CSB-EP503212FPG
CSB-BP503212FPG
CSB-MP503212FPG
CSB-EP503212FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Translation initiation factor 5A (eIF5A) CSB-YP503213FPG
CSB-EP503213FPG
CSB-BP503213FPG
CSB-MP503213FPG
CSB-EP503213FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Signal recognition 54 kDa protein (srp54) CSB-YP503214FPG
CSB-EP503214FPG
CSB-BP503214FPG
CSB-MP503214FPG
CSB-EP503214FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Chromatin protein Cren7 (creN7) CSB-YP503215FPG
CSB-EP503215FPG
CSB-BP503215FPG
CSB-MP503215FPG
CSB-EP503215FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Uridylate kinase (pyrH) CSB-YP503216FPG
CSB-EP503216FPG
CSB-BP503216FPG
CSB-MP503216FPG
CSB-EP503216FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Putative homocitrate synthase (YN1551_1618) CSB-YP503217FPG
CSB-EP503217FPG
CSB-BP503217FPG
CSB-MP503217FPG
CSB-EP503217FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus CCA-adding enzyme (cca) CSB-YP503218FPG
CSB-EP503218FPG
CSB-BP503218FPG
CSB-MP503218FPG
CSB-EP503218FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0200 protein YN1551_1675 (YN1551_1675) CSB-YP503219FPG
CSB-EP503219FPG
CSB-BP503219FPG
CSB-MP503219FPG
CSB-EP503219FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0201 protein YN1551_1676 (YN1551_1676) CSB-YP503220FPG
CSB-EP503220FPG
CSB-BP503220FPG
CSB-MP503220FPG
CSB-EP503220FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Ribonuclease Z (rnz) CSB-YP503221FPG
CSB-EP503221FPG
CSB-BP503221FPG
CSB-MP503221FPG
CSB-EP503221FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable DNA primase small subunit (priA) CSB-YP503222FPG
CSB-EP503222FPG
CSB-BP503222FPG
CSB-MP503222FPG
CSB-EP503222FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Translation initiation factor 2 subunit alpha (eIF2A) CSB-YP503223FPG
CSB-EP503223FPG
CSB-BP503223FPG
CSB-MP503223FPG
CSB-EP503223FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Ribosome biogenesis protein Nop10 (YN1551_1687) CSB-YP503224FPG
CSB-EP503224FPG
CSB-BP503224FPG
CSB-MP503224FPG
CSB-EP503224FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0286 protein YN1551_0025 (YN1551_0025) CSB-YP503225FPG
CSB-EP503225FPG
CSB-BP503225FPG
CSB-MP503225FPG
CSB-EP503225FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0284 protein YN1551_0030 (YN1551_0030) CSB-YP503226FPG
CSB-EP503226FPG
CSB-BP503226FPG
CSB-MP503226FPG
CSB-EP503226FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP503227FPG
CSB-EP503227FPG
CSB-BP503227FPG
CSB-MP503227FPG
CSB-EP503227FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Ribonuclease P protein component 4 (rnp4) CSB-YP503228FPG
CSB-EP503228FPG
CSB-BP503228FPG
CSB-MP503228FPG
CSB-EP503228FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable non-canonical purine NTP phosphatase (YN1551_0048) CSB-YP503229FPG
CSB-EP503229FPG
CSB-BP503229FPG
CSB-MP503229FPG
CSB-EP503229FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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