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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sulfolobus islandicus tRNA-splicing endonuclease (endA) CSB-YP503150FPG
CSB-EP503150FPG
CSB-BP503150FPG
CSB-MP503150FPG
CSB-EP503150FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus DNA-directed RNA polymerase subunit P (rpoP) CSB-YP503151FPG
CSB-EP503151FPG
CSB-BP503151FPG
CSB-MP503151FPG
CSB-EP503151FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Putative adenylate kinase (YN1551_1167) CSB-YP503152FPG
CSB-EP503152FPG
CSB-BP503152FPG
CSB-MP503152FPG
CSB-EP503152FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Arginine decarboxylase proenzyme (YN1551_1244) CSB-YP503153FPG
CSB-EP503153FPG
CSB-BP503153FPG
CSB-MP503153FPG
CSB-EP503153FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0219 protein YN1551_1245 (YN1551_1245) CSB-YP503154FPG
CSB-EP503154FPG
CSB-BP503154FPG
CSB-MP503154FPG
CSB-EP503154FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable ribosome biogenesis protein YN1551_1255 (YN1551_1255) CSB-YP503155FPG
CSB-EP503155FPG
CSB-BP503155FPG
CSB-MP503155FPG
CSB-EP503155FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus V-type proton ATPase subunit E (atpE) CSB-YP503156FPG
CSB-EP503156FPG
CSB-BP503156FPG
CSB-MP503156FPG
CSB-EP503156FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus V-type ATP synthase alpha chain (atpA), partial CSB-YP503157FPG
CSB-EP503157FPG
CSB-BP503157FPG
CSB-MP503157FPG
CSB-EP503157FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus V-type ATP synthase beta chain (atpB) CSB-YP503158FPG
CSB-EP503158FPG
CSB-BP503158FPG
CSB-MP503158FPG
CSB-EP503158FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus V-type ATP synthase subunit D (atpD) CSB-YP503159FPG
CSB-EP503159FPG
CSB-BP503159FPG
CSB-MP503159FPG
CSB-EP503159FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Proline--tRNA ligase (proS) CSB-YP503160FPG
CSB-EP503160FPG
CSB-BP503160FPG
CSB-MP503160FPG
CSB-EP503160FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP503161FPG
CSB-EP503161FPG
CSB-BP503161FPG
CSB-MP503161FPG
CSB-EP503161FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP503162FPG
CSB-EP503162FPG
CSB-BP503162FPG
CSB-MP503162FPG
CSB-EP503162FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Ketol-acid reductoisomerase (ilvC) CSB-YP503163FPG
CSB-EP503163FPG
CSB-BP503163FPG
CSB-MP503163FPG
CSB-EP503163FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP503164FPG
CSB-EP503164FPG
CSB-BP503164FPG
CSB-MP503164FPG
CSB-EP503164FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP503165FPG
CSB-EP503165FPG
CSB-BP503165FPG
CSB-MP503165FPG
CSB-EP503165FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Serine--tRNA ligase (serS) CSB-YP503166FPG
CSB-EP503166FPG
CSB-BP503166FPG
CSB-MP503166FPG
CSB-EP503166FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP503167FPG
CSB-EP503167FPG
CSB-BP503167FPG
CSB-MP503167FPG
CSB-EP503167FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Aspartate carbamoyltransferase (pyrB) CSB-YP503168FPG
CSB-EP503168FPG
CSB-BP503168FPG
CSB-MP503168FPG
CSB-EP503168FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP503169FPG
CSB-EP503169FPG
CSB-BP503169FPG
CSB-MP503169FPG
CSB-EP503169FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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