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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Sulfolobus islandicus 30S ribosomal protein S3Ae (rps3Ae) CSB-YP503190FPG
CSB-EP503190FPG
CSB-BP503190FPG
CSB-MP503190FPG
CSB-EP503190FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus 50S ribosomal protein L21e (rpl21e) CSB-YP503191FPG
CSB-EP503191FPG
CSB-BP503191FPG
CSB-MP503191FPG
CSB-EP503191FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable spermidine synthase (speE) CSB-YP503192FPG
CSB-EP503192FPG
CSB-BP503192FPG
CSB-MP503192FPG
CSB-EP503192FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus 50S ribosomal protein L37e (rpl37e) CSB-YP503193FPG
CSB-EP503193FPG
CSB-BP503193FPG
CSB-MP503193FPG
CSB-EP503193FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP503194FPG
CSB-EP503194FPG
CSB-BP503194FPG
CSB-MP503194FPG
CSB-EP503194FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Proteasome subunit beta 2 (psmB2) CSB-YP503195FPG
CSB-EP503195FPG
CSB-BP503195FPG
CSB-MP503195FPG
CSB-EP503195FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Replication factor C large subunit (rfcL) CSB-YP503196FPG
CSB-EP503196FPG
CSB-BP503196FPG
CSB-MP503196FPG
CSB-EP503196FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0290 protein YN1551_1483 (YN1551_1483), partial CSB-YP503197FPG1
CSB-EP503197FPG1
CSB-BP503197FPG1
CSB-MP503197FPG1
CSB-EP503197FPG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0148 protein YN1551_1488 (YN1551_1488) CSB-YP503198FPG
CSB-EP503198FPG
CSB-BP503198FPG
CSB-MP503198FPG
CSB-EP503198FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0147 protein YN1551_1489 (YN1551_1489) CSB-YP503199FPG
CSB-EP503199FPG
CSB-BP503199FPG
CSB-MP503199FPG
CSB-EP503199FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Anthranilate phosphoribosyltransferase (trpD) CSB-YP503200FPG
CSB-EP503200FPG
CSB-BP503200FPG
CSB-MP503200FPG
CSB-EP503200FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable N-glycosylase/DNA lyase (ogg) CSB-YP503201FPG
CSB-EP503201FPG
CSB-BP503201FPG
CSB-MP503201FPG
CSB-EP503201FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Enolase (eno) CSB-YP503202FPG
CSB-EP503202FPG
CSB-BP503202FPG
CSB-MP503202FPG
CSB-EP503202FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB), partial CSB-YP503203FPG
CSB-EP503203FPG
CSB-BP503203FPG
CSB-MP503203FPG
CSB-EP503203FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP503204FPG
CSB-EP503204FPG
CSB-BP503204FPG
CSB-MP503204FPG
CSB-EP503204FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Glutamyl-tRNA (Gln) amidotransferase subunit E, partial CSB-YP503205FPG
CSB-EP503205FPG
CSB-BP503205FPG
CSB-MP503205FPG
CSB-EP503205FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Glutamyl-tRNA (Gln) amidotransferase subunit D CSB-YP503206FPG
CSB-EP503206FPG
CSB-BP503206FPG
CSB-MP503206FPG
CSB-EP503206FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase (flpA) CSB-YP503207FPG
CSB-EP503207FPG
CSB-BP503207FPG
CSB-MP503207FPG
CSB-EP503207FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Putative HTH-type transcriptional regulatory protein YN1551_1579 (YN1551_1579) CSB-YP503208FPG
CSB-EP503208FPG
CSB-BP503208FPG
CSB-MP503208FPG
CSB-EP503208FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus tRNA (guanine (26)-N (2))-dimethyltransferase CSB-YP503209FPG
CSB-EP503209FPG
CSB-BP503209FPG
CSB-MP503209FPG
CSB-EP503209FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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