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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Sulfolobus islandicus D-tyrosyl-tRNA (Tyr) deacylase CSB-YP503230FPG
CSB-EP503230FPG
CSB-BP503230FPG
CSB-MP503230FPG
CSB-EP503230FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Phosphoenolpyruvate carboxylase (ppcA), partial CSB-YP503231FPG
CSB-EP503231FPG
CSB-BP503231FPG
CSB-MP503231FPG
CSB-EP503231FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable cobalt-precorrin-6Y C (15)-methyltransferase [decarboxylating] CSB-YP503232FPG
CSB-EP503232FPG
CSB-BP503232FPG
CSB-MP503232FPG
CSB-EP503232FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP503233FPG
CSB-EP503233FPG
CSB-BP503233FPG
CSB-MP503233FPG
CSB-EP503233FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Undecaprenyl-diphosphatase (uppP), partial CSB-YP503234FPG1
CSB-EP503234FPG1
CSB-BP503234FPG1
CSB-MP503234FPG1
CSB-EP503234FPG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Pyrrolidone-carboxylate peptidase (pcp) CSB-YP503235FPG
CSB-EP503235FPG
CSB-BP503235FPG
CSB-MP503235FPG
CSB-EP503235FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP503236FPG
CSB-EP503236FPG
CSB-BP503236FPG
CSB-MP503236FPG
CSB-EP503236FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Homoserine kinase (thrB) CSB-YP503237FPG
CSB-EP503237FPG
CSB-BP503237FPG
CSB-MP503237FPG
CSB-EP503237FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Translation initiation factor 1A (eIF1A) CSB-YP503238FPG
CSB-EP503238FPG
CSB-BP503238FPG
CSB-MP503238FPG
CSB-EP503238FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Translation initiation factor 2 subunit beta (eIF2B) CSB-YP503239FPG
CSB-EP503239FPG
CSB-BP503239FPG
CSB-MP503239FPG
CSB-EP503239FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP503240FPG
CSB-EP503240FPG
CSB-BP503240FPG
CSB-MP503240FPG
CSB-EP503240FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Protease HtpX homolog (htpX), partial CSB-YP503241FPG1
CSB-EP503241FPG1
CSB-BP503241FPG1
CSB-MP503241FPG1
CSB-EP503241FPG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus GMP synthase [glutamine-hydrolyzing] subunit B (guaAB) CSB-YP503242FPG
CSB-EP503242FPG
CSB-BP503242FPG
CSB-MP503242FPG
CSB-EP503242FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Cobalamin synthase (cobS), partial CSB-YP503243FPG1
CSB-EP503243FPG1
CSB-BP503243FPG1
CSB-MP503243FPG1
CSB-EP503243FPG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0282 protein YN1551_0671 (YN1551_0671) CSB-YP503244FPG
CSB-EP503244FPG
CSB-BP503244FPG
CSB-MP503244FPG
CSB-EP503244FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP503245FPG
CSB-EP503245FPG
CSB-BP503245FPG
CSB-MP503245FPG
CSB-EP503245FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0103 protein YN1551_0739 (YN1551_0739) CSB-YP503246FPG
CSB-EP503246FPG
CSB-BP503246FPG
CSB-MP503246FPG
CSB-EP503246FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus DNA-directed RNA polymerase subunit N (rpoN) CSB-YP503247FPG
CSB-EP503247FPG
CSB-BP503247FPG
CSB-MP503247FPG
CSB-EP503247FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus DNA-directed RNA polymerase subunit D (rpoD) CSB-YP503248FPG
CSB-EP503248FPG
CSB-BP503248FPG
CSB-MP503248FPG
CSB-EP503248FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus 30S ribosomal protein S4P (rps4p) CSB-YP503249FPG
CSB-EP503249FPG
CSB-BP503249FPG
CSB-MP503249FPG
CSB-EP503249FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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