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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus anthracis 3'-5' exoribonuclease yhaM (yhaM) CSB-YP503310BQF
CSB-EP503310BQF
CSB-BP503310BQF
CSB-MP503310BQF
CSB-EP503310BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Glycerol kinase (glpK) CSB-YP503311BQF
CSB-EP503311BQF
CSB-BP503311BQF
CSB-MP503311BQF
CSB-EP503311BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis HTH-type transcriptional regulator hpr (hpr) CSB-YP503312BQF
CSB-EP503312BQF
CSB-BP503312BQF
CSB-MP503312BQF
CSB-EP503312BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0316 protein BAA_3454 (BAA_3454), partial CSB-YP503313BQF1
CSB-EP503313BQF1
CSB-BP503313BQF1
CSB-MP503313BQF1
CSB-EP503313BQF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0303 protein BAA_3481 (BAA_3481) CSB-YP503314BQF
CSB-EP503314BQF
CSB-BP503314BQF
CSB-MP503314BQF
CSB-EP503314BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 50S ribosomal protein L9 (rplI) CSB-YP503315BQF
CSB-EP503315BQF
CSB-BP503315BQF
CSB-MP503315BQF
CSB-EP503315BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 30S ribosomal protein S18 (rpsR) CSB-YP503316BQF
CSB-EP503316BQF
CSB-BP503316BQF
CSB-MP503316BQF
CSB-EP503316BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 30S ribosomal protein S6 (rpsF) CSB-YP503317BQF
CSB-EP503317BQF
CSB-BP503317BQF
CSB-MP503317BQF
CSB-EP503317BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Nucleoid occlusion protein (noc) CSB-YP503318BQF
CSB-EP503318BQF
CSB-BP503318BQF
CSB-MP503318BQF
CSB-EP503318BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP503319BQF
CSB-EP503319BQF
CSB-BP503319BQF
CSB-MP503319BQF
CSB-EP503319BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Ribonuclease P protein component (rnpA) CSB-YP503320BQF
CSB-EP503320BQF
CSB-BP503320BQF
CSB-MP503320BQF
CSB-EP503320BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 50S ribosomal protein L34 (rpmH) CSB-YP503321BQF
CSB-EP503321BQF
CSB-BP503321BQF
CSB-MP503321BQF
CSB-EP503321BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0344 protein BAA_1237 (BAA_1237), partial CSB-YP503322BQF1
CSB-EP503322BQF1
CSB-BP503322BQF1
CSB-MP503322BQF1
CSB-EP503322BQF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0234 protein BAA_1246 (BAA_1246) CSB-YP503323BQF
CSB-EP503323BQF
CSB-BP503323BQF
CSB-MP503323BQF
CSB-EP503323BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0736 protein BAA_1264 (BAA_1264) CSB-YP503324BQF
CSB-EP503324BQF
CSB-BP503324BQF
CSB-MP503324BQF
CSB-EP503324BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0738 protein BAA_1286 (BAA_1286) CSB-YP503325BQF
CSB-EP503325BQF
CSB-BP503325BQF
CSB-MP503325BQF
CSB-EP503325BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Bis (5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] CSB-YP503326BQF
CSB-EP503326BQF
CSB-BP503326BQF
CSB-MP503326BQF
CSB-EP503326BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0477 protein BAA_1317 (BAA_1317) CSB-YP503327BQF
CSB-EP503327BQF
CSB-BP503327BQF
CSB-MP503327BQF
CSB-EP503327BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Anthranilate phosphoribosyltransferase (trpD) CSB-YP503328BQF
CSB-EP503328BQF
CSB-BP503328BQF
CSB-MP503328BQF
CSB-EP503328BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Indole-3-glycerol phosphate synthase (trpC) CSB-YP503329BQF
CSB-EP503329BQF
CSB-BP503329BQF
CSB-MP503329BQF
CSB-EP503329BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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