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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Sulfolobus islandicus Proline--tRNA ligase (proS) CSB-YP503090FPF
CSB-EP503090FPF
CSB-BP503090FPF
CSB-MP503090FPF
CSB-EP503090FPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus V-type ATP synthase subunit D (atpD) CSB-YP503091FPF
CSB-EP503091FPF
CSB-BP503091FPF
CSB-MP503091FPF
CSB-EP503091FPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus V-type ATP synthase beta chain (atpB) CSB-YP503092FPF
CSB-EP503092FPF
CSB-BP503092FPF
CSB-MP503092FPF
CSB-EP503092FPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus V-type ATP synthase alpha chain (atpA), partial CSB-YP503093FPF
CSB-EP503093FPF
CSB-BP503093FPF
CSB-MP503093FPF
CSB-EP503093FPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus V-type proton ATPase subunit E (atpE) CSB-YP503094FPF
CSB-EP503094FPF
CSB-BP503094FPF
CSB-MP503094FPF
CSB-EP503094FPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable ribosome biogenesis protein YG5714_1581 (YG5714_1581) CSB-YP503095FPF
CSB-EP503095FPF
CSB-BP503095FPF
CSB-MP503095FPF
CSB-EP503095FPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0219 protein YG5714_1591 (YG5714_1591) CSB-YP503096FPF
CSB-EP503096FPF
CSB-BP503096FPF
CSB-MP503096FPF
CSB-EP503096FPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Arginine decarboxylase proenzyme (YG5714_1592) CSB-YP503097FPF
CSB-EP503097FPF
CSB-BP503097FPF
CSB-MP503097FPF
CSB-EP503097FPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Acetylglutamate/acetylaminoadipate kinase (argB) CSB-YP503098FPG
CSB-EP503098FPG
CSB-BP503098FPG
CSB-MP503098FPG
CSB-EP503098FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Acetyl-lysine deacetylase (lysK) CSB-YP503099FPG
CSB-EP503099FPG
CSB-BP503099FPG
CSB-MP503099FPG
CSB-EP503099FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus 30S ribosomal protein S8e (rps8e) CSB-YP503100FPG
CSB-EP503100FPG
CSB-BP503100FPG
CSB-MP503100FPG
CSB-EP503100FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus 50S ribosomal protein L40e (rpl40e) CSB-YP503101FPG
CSB-EP503101FPG
CSB-BP503101FPG
CSB-MP503101FPG
CSB-EP503101FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Peptidyl-tRNA hydrolase (pth) CSB-YP503102FPG
CSB-EP503102FPG
CSB-BP503102FPG
CSB-MP503102FPG
CSB-EP503102FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Elongation factor 1-beta (ef1b) CSB-YP503103FPG
CSB-EP503103FPG
CSB-BP503103FPG
CSB-MP503103FPG
CSB-EP503103FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Glutamyl-tRNA reductase (hemA) CSB-YP503104FPG
CSB-EP503104FPG
CSB-BP503104FPG
CSB-MP503104FPG
CSB-EP503104FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP503105FPG
CSB-EP503105FPG
CSB-BP503105FPG
CSB-MP503105FPG
CSB-EP503105FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable porphobilinogen deaminase (hemC) CSB-YP503106FPG
CSB-EP503106FPG
CSB-BP503106FPG
CSB-MP503106FPG
CSB-EP503106FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus DNA ligase (lig), partial CSB-YP503107FPG
CSB-EP503107FPG
CSB-BP503107FPG
CSB-MP503107FPG
CSB-EP503107FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Protein YN1551_0855 (YN1551_0855) CSB-YP503108FPG
CSB-EP503108FPG
CSB-BP503108FPG
CSB-MP503108FPG
CSB-EP503108FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus S-adenosylmethionine synthase (mat) CSB-YP503109FPG
CSB-EP503109FPG
CSB-BP503109FPG
CSB-MP503109FPG
CSB-EP503109FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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