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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Amoebophilus asiaticus DNA ligase (ligA), partial CSB-YP501430BYI
CSB-EP501430BYI
CSB-BP501430BYI
CSB-MP501430BYI
CSB-EP501430BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Redox-sensing transcriptional repressor rex (rex) CSB-YP501431BQG
CSB-EP501431BQG
CSB-BP501431BQG
CSB-MP501431BQG
CSB-EP501431BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 10 kDa chaperonin (groS) CSB-YP501432BQG
CSB-EP501432BQG
CSB-BP501432BQG
CSB-MP501432BQG
CSB-EP501432BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 60 kDa chaperonin (groL), partial CSB-YP501433BQG
CSB-EP501433BQG
CSB-BP501433BQG
CSB-MP501433BQG
CSB-EP501433BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP501434BQG
CSB-EP501434BQG
CSB-BP501434BQG
CSB-MP501434BQG
CSB-EP501434BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP501435BQG
CSB-EP501435BQG
CSB-BP501435BQG
CSB-MP501435BQG
CSB-EP501435BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP501436BQG
CSB-EP501436BQG
CSB-BP501436BQG
CSB-MP501436BQG
CSB-EP501436BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP501437BQG
CSB-EP501437BQG
CSB-BP501437BQG
CSB-MP501437BQG
CSB-EP501437BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP501438BQG
CSB-EP501438BQG
CSB-BP501438BQG
CSB-MP501438BQG
CSB-EP501438BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP501439BQG
CSB-EP501439BQG
CSB-BP501439BQG
CSB-MP501439BQG
CSB-EP501439BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis DNA ligase (ligA), partial CSB-YP501440BQG
CSB-EP501440BQG
CSB-BP501440BQG
CSB-MP501440BQG
CSB-EP501440BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP501441BQG
CSB-EP501441BQG
CSB-BP501441BQG
CSB-MP501441BQG
CSB-EP501441BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP501442BQG
CSB-EP501442BQG
CSB-BP501442BQG
CSB-MP501442BQG
CSB-EP501442BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP501443BQG
CSB-EP501443BQG
CSB-BP501443BQG
CSB-MP501443BQG
CSB-EP501443BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP501444BQG
CSB-EP501444BQG
CSB-BP501444BQG
CSB-MP501444BQG
CSB-EP501444BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Metallothiol transferase fosB 1 (fosB1) CSB-YP501445BQG
CSB-EP501445BQG
CSB-BP501445BQG
CSB-MP501445BQG
CSB-EP501445BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis NADPH dehydrogenase (namA) CSB-YP501446BQG
CSB-EP501446BQG
CSB-BP501446BQG
CSB-MP501446BQG
CSB-EP501446BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis NH (3)-dependent NAD (+) synthetase CSB-YP501447BQG
CSB-EP501447BQG
CSB-BP501447BQG
CSB-MP501447BQG
CSB-EP501447BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Homoserine kinase (thrB) CSB-YP501448BQG
CSB-EP501448BQG
CSB-BP501448BQG
CSB-MP501448BQG
CSB-EP501448BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis DNA repair protein recO (recO) CSB-YP501449BQG
CSB-EP501449BQG
CSB-BP501449BQG
CSB-MP501449BQG
CSB-EP501449BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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