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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus anthracis Lactate utilization protein A 1 (lutA1) CSB-YP501630BQG
CSB-EP501630BQG
CSB-BP501630BQG
CSB-MP501630BQG
CSB-EP501630BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis HTH-type transcriptional regulator hpr (hpr) CSB-YP501631BQG
CSB-EP501631BQG
CSB-BP501631BQG
CSB-MP501631BQG
CSB-EP501631BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Glycerol kinase (glpK) CSB-YP501632BQG
CSB-EP501632BQG
CSB-BP501632BQG
CSB-MP501632BQG
CSB-EP501632BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 3'-5' exoribonuclease yhaM (yhaM) CSB-YP501633BQG
CSB-EP501633BQG
CSB-BP501633BQG
CSB-MP501633BQG
CSB-EP501633BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Serine-protein kinase rsbW (rsbW) CSB-YP501634BQG
CSB-EP501634BQG
CSB-BP501634BQG
CSB-MP501634BQG
CSB-EP501634BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Methionine--tRNA ligase (metG), partial CSB-YP501635BQG
CSB-EP501635BQG
CSB-BP501635BQG
CSB-MP501635BQG
CSB-EP501635BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis SsrA-binding protein (smpB) CSB-YP501636BQG
CSB-EP501636BQG
CSB-BP501636BQG
CSB-MP501636BQG
CSB-EP501636BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Enolase (eno) CSB-YP501637BQG
CSB-EP501637BQG
CSB-BP501637BQG
CSB-MP501637BQG
CSB-EP501637BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoglycerate kinase (pgk) CSB-YP501638BQG
CSB-EP501638BQG
CSB-BP501638BQG
CSB-MP501638BQG
CSB-EP501638BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Protein ArsC (arsC) CSB-YP501639BQG
CSB-EP501639BQG
CSB-BP501639BQG
CSB-MP501639BQG
CSB-EP501639BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0753 protein BAMEG_1429 (BAMEG_1429), partial CSB-YP501640BQG
CSB-EP501640BQG
CSB-BP501640BQG
CSB-MP501640BQG
CSB-EP501640BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0271 protein BAMEG_1513 (BAMEG_1513) CSB-YP501641BQG
CSB-EP501641BQG
CSB-BP501641BQG
CSB-MP501641BQG
CSB-EP501641BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Pyrrolidone-carboxylate peptidase (pcp) CSB-YP501642BQG
CSB-EP501642BQG
CSB-BP501642BQG
CSB-MP501642BQG
CSB-EP501642BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Putative 3-methyladenine DNA glycosylase (BAMEG_3690) CSB-YP501643BQG
CSB-EP501643BQG
CSB-BP501643BQG
CSB-MP501643BQG
CSB-EP501643BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0342 protein BAMEG_3696 (BAMEG_3696) CSB-YP501644BQG
CSB-EP501644BQG
CSB-BP501644BQG
CSB-MP501644BQG
CSB-EP501644BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0754 membrane protein BAMEG_3697 (BAMEG_3697), partial CSB-YP501645BQG1
CSB-EP501645BQG1
CSB-BP501645BQG1
CSB-MP501645BQG1
CSB-EP501645BQG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP501646BQG
CSB-EP501646BQG
CSB-BP501646BQG
CSB-MP501646BQG
CSB-EP501646BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Putative sporulation transcription regulator WhiA (whiA) CSB-YP501647BQG
CSB-EP501647BQG
CSB-BP501647BQG
CSB-MP501647BQG
CSB-EP501647BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0042 nucleotide-binding protein BAMEG_5437 (BAMEG_5437) CSB-YP501648BQG
CSB-EP501648BQG
CSB-BP501648BQG
CSB-MP501648BQG
CSB-EP501648BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Pyrophosphatase ppaX (ppaX) CSB-YP501649BQG
CSB-EP501649BQG
CSB-BP501649BQG
CSB-MP501649BQG
CSB-EP501649BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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