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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus anthracis Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP501570BQG
CSB-EP501570BQG
CSB-BP501570BQG
CSB-MP501570BQG
CSB-EP501570BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis GTPase Der (der) CSB-YP501571BQG
CSB-EP501571BQG
CSB-BP501571BQG
CSB-MP501571BQG
CSB-EP501571BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP501572BQG
CSB-EP501572BQG
CSB-BP501572BQG
CSB-MP501572BQG
CSB-EP501572BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Cytidylate kinase (cmk) CSB-YP501573BQG
CSB-EP501573BQG
CSB-BP501573BQG
CSB-MP501573BQG
CSB-EP501573BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP501574BQG
CSB-EP501574BQG
CSB-BP501574BQG
CSB-MP501574BQG
CSB-EP501574BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Sulfate adenylyltransferase (sat) CSB-YP501575BQG
CSB-EP501575BQG
CSB-BP501575BQG
CSB-MP501575BQG
CSB-EP501575BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP501576BQG
CSB-EP501576BQG
CSB-BP501576BQG
CSB-MP501576BQG
CSB-EP501576BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP501577BQG
CSB-EP501577BQG
CSB-BP501577BQG
CSB-MP501577BQG
CSB-EP501577BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP501578BQG
CSB-EP501578BQG
CSB-BP501578BQG
CSB-MP501578BQG
CSB-EP501578BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP501579BQG
CSB-EP501579BQG
CSB-BP501579BQG
CSB-MP501579BQG
CSB-EP501579BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP501580BQG
CSB-EP501580BQG
CSB-BP501580BQG
CSB-MP501580BQG
CSB-EP501580BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP501581BQG
CSB-EP501581BQG
CSB-BP501581BQG
CSB-MP501581BQG
CSB-EP501581BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP501582BQG
CSB-EP501582BQG
CSB-BP501582BQG
CSB-MP501582BQG
CSB-EP501582BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis ATP phosphoribosyltransferase (hisG) CSB-YP501583BQG
CSB-EP501583BQG
CSB-BP501583BQG
CSB-MP501583BQG
CSB-EP501583BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP501584BQG
CSB-EP501584BQG
CSB-BP501584BQG
CSB-MP501584BQG
CSB-EP501584BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP501585BQG
CSB-EP501585BQG
CSB-BP501585BQG
CSB-MP501585BQG
CSB-EP501585BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP501586BQG
CSB-EP501586BQG
CSB-BP501586BQG
CSB-MP501586BQG
CSB-EP501586BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 2-isopropylmalate synthase (leuA), partial CSB-YP501587BQG
CSB-EP501587BQG
CSB-BP501587BQG
CSB-MP501587BQG
CSB-EP501587BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0180 protein BAMEG_3182 (BAMEG_3182) CSB-YP501588BQG
CSB-EP501588BQG
CSB-BP501588BQG
CSB-MP501588BQG
CSB-EP501588BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Probable tRNA sulfurtransferase (thiI) CSB-YP501589BQG
CSB-EP501589BQG
CSB-BP501589BQG
CSB-MP501589BQG
CSB-EP501589BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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