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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus anthracis Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP501530BQG
CSB-EP501530BQG
CSB-BP501530BQG
CSB-MP501530BQG
CSB-EP501530BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Putative competence-damage inducible protein (cinA) CSB-YP501531BQG
CSB-EP501531BQG
CSB-BP501531BQG
CSB-MP501531BQG
CSB-EP501531BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Probable D-serine dehydratase (dsdA) CSB-YP501532BQG
CSB-EP501532BQG
CSB-BP501532BQG
CSB-MP501532BQG
CSB-EP501532BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Ribonuclease PH (rph) CSB-YP501533BQG
CSB-EP501533BQG
CSB-BP501533BQG
CSB-MP501533BQG
CSB-EP501533BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UvrABC system protein C (uvrC), partial CSB-YP501534BQG
CSB-EP501534BQG
CSB-BP501534BQG
CSB-MP501534BQG
CSB-EP501534BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Heme-degrading monooxygenase isdG (isdG) CSB-YP501535BQG
CSB-EP501535BQG
CSB-BP501535BQG
CSB-MP501535BQG
CSB-EP501535BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis MutS2 protein (mutS2), partial CSB-YP501536BQG
CSB-EP501536BQG
CSB-BP501536BQG
CSB-MP501536BQG
CSB-EP501536BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis DNA mismatch repair protein MutS (mutS), partial CSB-YP501537BQG
CSB-EP501537BQG
CSB-BP501537BQG
CSB-MP501537BQG
CSB-EP501537BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis DNA mismatch repair protein mutL (mutL), partial CSB-YP501538BQG
CSB-EP501538BQG
CSB-BP501538BQG
CSB-MP501538BQG
CSB-EP501538BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Peptidase T (pepT) CSB-YP501539BQG
CSB-EP501539BQG
CSB-BP501539BQG
CSB-MP501539BQG
CSB-EP501539BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Protein hfq (hfq) CSB-YP501540BQG
CSB-EP501540BQG
CSB-BP501540BQG
CSB-MP501540BQG
CSB-EP501540BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Xanthine phosphoribosyltransferase (xpt) CSB-YP501541BQG
CSB-EP501541BQG
CSB-BP501541BQG
CSB-MP501541BQG
CSB-EP501541BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Cell cycle protein GpsB (gpsB) CSB-YP501542BQG
CSB-EP501542BQG
CSB-BP501542BQG
CSB-MP501542BQG
CSB-EP501542BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0398 protein BAMEG_3014 (BAMEG_3014) CSB-YP501543BQG
CSB-EP501543BQG
CSB-BP501543BQG
CSB-MP501543BQG
CSB-EP501543BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Holliday junction resolvase recU (recU) CSB-YP501544BQG
CSB-EP501544BQG
CSB-BP501544BQG
CSB-MP501544BQG
CSB-EP501544BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Aspartate 1-decarboxylase (panD) CSB-YP501545BQG
CSB-EP501545BQG
CSB-BP501545BQG
CSB-MP501545BQG
CSB-EP501545BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Pantothenate synthetase (panC) CSB-YP501546BQG
CSB-EP501546BQG
CSB-BP501546BQG
CSB-MP501546BQG
CSB-EP501546BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP501547BQG
CSB-EP501547BQG
CSB-BP501547BQG
CSB-MP501547BQG
CSB-EP501547BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis CCA-adding enzyme (cca) CSB-YP501548BQG
CSB-EP501548BQG
CSB-BP501548BQG
CSB-MP501548BQG
CSB-EP501548BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Methylglyoxal synthase (mgsA) CSB-YP501549BQG
CSB-EP501549BQG
CSB-BP501549BQG
CSB-MP501549BQG
CSB-EP501549BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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