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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus anthracis Dihydrodipicolinate reductase (dapB) CSB-YP501550BQG
CSB-EP501550BQG
CSB-BP501550BQG
CSB-MP501550BQG
CSB-EP501550BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0302 protein BAMEG_3052 (BAMEG_3052) CSB-YP501551BQG
CSB-EP501551BQG
CSB-BP501551BQG
CSB-MP501551BQG
CSB-EP501551BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 3-dehydroquinate synthase (aroB) CSB-YP501552BQG
CSB-EP501552BQG
CSB-BP501552BQG
CSB-MP501552BQG
CSB-EP501552BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Demethylmenaquinone methyltransferase (ubiE) CSB-YP501553BQG
CSB-EP501553BQG
CSB-BP501553BQG
CSB-MP501553BQG
CSB-EP501553BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Ribonuclease HIII (rnhC) CSB-YP501554BQG
CSB-EP501554BQG
CSB-BP501554BQG
CSB-MP501554BQG
CSB-EP501554BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP501555BQG
CSB-EP501555BQG
CSB-BP501555BQG
CSB-MP501555BQG
CSB-EP501555BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Small, acid-soluble spore protein I (sspI) CSB-YP501556BQG
CSB-EP501556BQG
CSB-BP501556BQG
CSB-MP501556BQG
CSB-EP501556BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 50S ribosomal protein L20 (rplT) CSB-YP501557BQG
CSB-EP501557BQG
CSB-BP501557BQG
CSB-MP501557BQG
CSB-EP501557BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 50S ribosomal protein L35 (rpmI) CSB-YP501558BQG
CSB-EP501558BQG
CSB-BP501558BQG
CSB-MP501558BQG
CSB-EP501558BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Transcriptional repressor NrdR (nrdR) CSB-YP501559BQG
CSB-EP501559BQG
CSB-BP501559BQG
CSB-MP501559BQG
CSB-EP501559BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0756 membrane protein BAMEG_4871 (BAMEG_4871), partial CSB-YP501560BQG1
CSB-EP501560BQG1
CSB-BP501560BQG1
CSB-MP501560BQG1
CSB-EP501560BQG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP501561BQG
CSB-EP501561BQG
CSB-BP501561BQG
CSB-MP501561BQG
CSB-EP501561BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP501562BQG
CSB-EP501562BQG
CSB-BP501562BQG
CSB-MP501562BQG
CSB-EP501562BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0173 metal-dependent hydrolase BAMEG_4892 (BAMEG_4892) CSB-YP501563BQG
CSB-EP501563BQG
CSB-BP501563BQG
CSB-MP501563BQG
CSB-EP501563BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0154 protein BAMEG_0893 (BAMEG_0893), partial CSB-YP501564BQG
CSB-EP501564BQG
CSB-BP501564BQG
CSB-MP501564BQG
CSB-EP501564BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Hut operon positive regulatory protein (hutP) CSB-YP501565BQG
CSB-EP501565BQG
CSB-BP501565BQG
CSB-MP501565BQG
CSB-EP501565BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Imidazolonepropionase (hutI) CSB-YP501566BQG
CSB-EP501566BQG
CSB-BP501566BQG
CSB-MP501566BQG
CSB-EP501566BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Small, acid-soluble spore protein O (sspO) CSB-YP501567BQG
CSB-EP501567BQG
CSB-BP501567BQG
CSB-MP501567BQG
CSB-EP501567BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Small, acid-soluble spore protein N (sspN) CSB-YP501568BQG
CSB-EP501568BQG
CSB-BP501568BQG
CSB-MP501568BQG
CSB-EP501568BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Small, acid-soluble spore protein tlp (tlp) CSB-YP501569BQG
CSB-EP501569BQG
CSB-BP501569BQG
CSB-MP501569BQG
CSB-EP501569BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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