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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Bacillus anthracis UPF0356 protein BAMEG_4229 (BAMEG_4229) CSB-YP501710BQG
CSB-EP501710BQG
CSB-BP501710BQG
CSB-MP501710BQG
CSB-EP501710BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis N-acetyldiaminopimelate deacetylase (BAMEG_4233) CSB-YP501711BQG
CSB-EP501711BQG
CSB-BP501711BQG
CSB-MP501711BQG
CSB-EP501711BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP501712BQG
CSB-EP501712BQG
CSB-BP501712BQG
CSB-MP501712BQG
CSB-EP501712BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP501713BQG
CSB-EP501713BQG
CSB-BP501713BQG
CSB-MP501713BQG
CSB-EP501713BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Methylthioribose kinase (mtnK) CSB-YP501714BQG
CSB-EP501714BQG
CSB-BP501714BQG
CSB-MP501714BQG
CSB-EP501714BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW) CSB-YP501715BQG
CSB-EP501715BQG
CSB-BP501715BQG
CSB-MP501715BQG
CSB-EP501715BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP501716BQG
CSB-EP501716BQG
CSB-BP501716BQG
CSB-MP501716BQG
CSB-EP501716BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP501717BQG
CSB-EP501717BQG
CSB-BP501717BQG
CSB-MP501717BQG
CSB-EP501717BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Chromosomal replication initiator protein DnaA (dnaA) CSB-YP501718BQG
CSB-EP501718BQG
CSB-BP501718BQG
CSB-MP501718BQG
CSB-EP501718BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis DNA replication and repair protein recF (recF) CSB-YP501719BQG
CSB-EP501719BQG
CSB-BP501719BQG
CSB-MP501719BQG
CSB-EP501719BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP501720BQG
CSB-EP501720BQG
CSB-BP501720BQG
CSB-MP501720BQG
CSB-EP501720BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP501721BQG
CSB-EP501721BQG
CSB-BP501721BQG
CSB-MP501721BQG
CSB-EP501721BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Glucosamine-6-phosphate deaminase (nagB) CSB-YP501722BQG
CSB-EP501722BQG
CSB-BP501722BQG
CSB-MP501722BQG
CSB-EP501722BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Segregation and condensation protein B (scpB) CSB-YP501723BQG
CSB-EP501723BQG
CSB-BP501723BQG
CSB-MP501723BQG
CSB-EP501723BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Segregation and condensation protein A (scpA) CSB-YP501724BQG
CSB-EP501724BQG
CSB-BP501724BQG
CSB-MP501724BQG
CSB-EP501724BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Anti-sigma F factor (spoIIAB) CSB-YP501725BQG
CSB-EP501725BQG
CSB-BP501725BQG
CSB-MP501725BQG
CSB-EP501725BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphopentomutase (deoB) CSB-YP501726BQG
CSB-EP501726BQG
CSB-BP501726BQG
CSB-MP501726BQG
CSB-EP501726BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP501727BQG
CSB-EP501727BQG
CSB-BP501727BQG
CSB-MP501727BQG
CSB-EP501727BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP501728BQG
CSB-EP501728BQG
CSB-BP501728BQG
CSB-MP501728BQG
CSB-EP501728BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP501729BQG
CSB-EP501729BQG
CSB-BP501729BQG
CSB-MP501729BQG
CSB-EP501729BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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