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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sulfolobus islandicus Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP502910FPD
CSB-EP502910FPD
CSB-BP502910FPD
CSB-MP502910FPD
CSB-EP502910FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Aspartate carbamoyltransferase (pyrB) CSB-YP502911FPD
CSB-EP502911FPD
CSB-BP502911FPD
CSB-MP502911FPD
CSB-EP502911FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP502912FPD
CSB-EP502912FPD
CSB-BP502912FPD
CSB-MP502912FPD
CSB-EP502912FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Serine--tRNA ligase (serS) CSB-YP502913FPD
CSB-EP502913FPD
CSB-BP502913FPD
CSB-MP502913FPD
CSB-EP502913FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP502914FPD
CSB-EP502914FPD
CSB-BP502914FPD
CSB-MP502914FPD
CSB-EP502914FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP502915FPD
CSB-EP502915FPD
CSB-BP502915FPD
CSB-MP502915FPD
CSB-EP502915FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Ketol-acid reductoisomerase (ilvC) CSB-YP502916FPD
CSB-EP502916FPD
CSB-BP502916FPD
CSB-MP502916FPD
CSB-EP502916FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP502917FPD
CSB-EP502917FPD
CSB-BP502917FPD
CSB-MP502917FPD
CSB-EP502917FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP502918FPD
CSB-EP502918FPD
CSB-BP502918FPD
CSB-MP502918FPD
CSB-EP502918FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Proline--tRNA ligase (proS) CSB-YP502919FPD
CSB-EP502919FPD
CSB-BP502919FPD
CSB-MP502919FPD
CSB-EP502919FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus V-type ATP synthase subunit D (atpD) CSB-YP502920FPD
CSB-EP502920FPD
CSB-BP502920FPD
CSB-MP502920FPD
CSB-EP502920FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus V-type ATP synthase beta chain (atpB) CSB-YP502921FPD
CSB-EP502921FPD
CSB-BP502921FPD
CSB-MP502921FPD
CSB-EP502921FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus V-type ATP synthase alpha chain (atpA), partial CSB-YP502922FPD
CSB-EP502922FPD
CSB-BP502922FPD
CSB-MP502922FPD
CSB-EP502922FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus V-type proton ATPase subunit E (atpE) CSB-YP502923FPD
CSB-EP502923FPD
CSB-BP502923FPD
CSB-MP502923FPD
CSB-EP502923FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable ribosome biogenesis protein M1627_1693 (M1627_1693) CSB-YP502924FPD
CSB-EP502924FPD
CSB-BP502924FPD
CSB-MP502924FPD
CSB-EP502924FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0219 protein M1627_1703 (M1627_1703) CSB-YP502925FPD
CSB-EP502925FPD
CSB-BP502925FPD
CSB-MP502925FPD
CSB-EP502925FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Arginine decarboxylase proenzyme (M1627_1704) CSB-YP502926FPD
CSB-EP502926FPD
CSB-BP502926FPD
CSB-MP502926FPD
CSB-EP502926FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Putative adenylate kinase (M1627_1760) CSB-YP502927FPD
CSB-EP502927FPD
CSB-BP502927FPD
CSB-MP502927FPD
CSB-EP502927FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus DNA-directed RNA polymerase subunit P (rpoP) CSB-YP502928FPD
CSB-EP502928FPD
CSB-BP502928FPD
CSB-MP502928FPD
CSB-EP502928FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus tRNA-splicing endonuclease (endA) CSB-YP502929FPD
CSB-EP502929FPD
CSB-BP502929FPD
CSB-MP502929FPD
CSB-EP502929FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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