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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sulfolobus islandicus D-tyrosyl-tRNA (Tyr) deacylase CSB-YP502830FPD
CSB-EP502830FPD
CSB-BP502830FPD
CSB-MP502830FPD
CSB-EP502830FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Phosphoenolpyruvate carboxylase (ppcA), partial CSB-YP502831FPD
CSB-EP502831FPD
CSB-BP502831FPD
CSB-MP502831FPD
CSB-EP502831FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable cobalt-precorrin-6Y C (15)-methyltransferase [decarboxylating] CSB-YP502832FPD
CSB-EP502832FPD
CSB-BP502832FPD
CSB-MP502832FPD
CSB-EP502832FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP502833FPD
CSB-EP502833FPD
CSB-BP502833FPD
CSB-MP502833FPD
CSB-EP502833FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Homoserine kinase (thrB) CSB-YP502834FPD
CSB-EP502834FPD
CSB-BP502834FPD
CSB-MP502834FPD
CSB-EP502834FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Translation initiation factor 1A (eIF1A) CSB-YP502835FPD
CSB-EP502835FPD
CSB-BP502835FPD
CSB-MP502835FPD
CSB-EP502835FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0282 protein M1627_2198 (M1627_2198) CSB-YP502836FPD
CSB-EP502836FPD
CSB-BP502836FPD
CSB-MP502836FPD
CSB-EP502836FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Cobalamin synthase (cobS), partial CSB-YP502837FPD1
CSB-EP502837FPD1
CSB-BP502837FPD1
CSB-MP502837FPD1
CSB-EP502837FPD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus GMP synthase [glutamine-hydrolyzing] subunit B (guaAB) CSB-YP502838FPD
CSB-EP502838FPD
CSB-BP502838FPD
CSB-MP502838FPD
CSB-EP502838FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Translation initiation factor 2 subunit beta (eIF2B) CSB-YP502839FPD
CSB-EP502839FPD
CSB-BP502839FPD
CSB-MP502839FPD
CSB-EP502839FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP502840FPD
CSB-EP502840FPD
CSB-BP502840FPD
CSB-MP502840FPD
CSB-EP502840FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus 50S ribosomal protein L13e (rpl13e) CSB-YP502841FPD
CSB-EP502841FPD
CSB-BP502841FPD
CSB-MP502841FPD
CSB-EP502841FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus RNA 3'-terminal phosphate cyclase (rtcA) CSB-YP502842FPD
CSB-EP502842FPD
CSB-BP502842FPD
CSB-MP502842FPD
CSB-EP502842FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Endonuclease V (nfi) CSB-YP502843FPD
CSB-EP502843FPD
CSB-BP502843FPD
CSB-MP502843FPD
CSB-EP502843FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP502844FPD
CSB-EP502844FPD
CSB-BP502844FPD
CSB-MP502844FPD
CSB-EP502844FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Threonine--tRNA ligase (thrS), partial CSB-YP502845FPD
CSB-EP502845FPD
CSB-BP502845FPD
CSB-MP502845FPD
CSB-EP502845FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP502846FPD
CSB-EP502846FPD
CSB-BP502846FPD
CSB-MP502846FPD
CSB-EP502846FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Putative antitoxin M1627_0365 (M1627_0365) CSB-YP502847FPD
CSB-EP502847FPD
CSB-BP502847FPD
CSB-MP502847FPD
CSB-EP502847FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Pyrrolidone-carboxylate peptidase (pcp) CSB-YP502848FPD
CSB-EP502848FPD
CSB-BP502848FPD
CSB-MP502848FPD
CSB-EP502848FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Glycerol kinase (glpK), partial CSB-YP502849FPD
CSB-EP502849FPD
CSB-BP502849FPD
CSB-MP502849FPD
CSB-EP502849FPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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