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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Dihydrodipicolinate reductase (dapB) CSB-YP509210ENU
CSB-EP509210ENU
CSB-BP509210ENU
CSB-MP509210ENU
CSB-EP509210ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein fixA (fixA) CSB-YP509211ENU
CSB-EP509211ENU
CSB-BP509211ENU
CSB-MP509211ENU
CSB-EP509211ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP509212ENU
CSB-EP509212ENU
CSB-BP509212ENU
CSB-MP509212ENU
CSB-EP509212ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Outer-membrane lipoprotein carrier protein (lolA) CSB-YP509213ENU
CSB-EP509213ENU
CSB-BP509213ENU
CSB-MP509213ENU
CSB-EP509213ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0434 protein ycaR (ycaR) CSB-YP509214ENU
CSB-EP509214ENU
CSB-BP509214ENU
CSB-MP509214ENU
CSB-EP509214ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP509215ENU
CSB-EP509215ENU
CSB-BP509215ENU
CSB-MP509215ENU
CSB-EP509215ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Chaperone modulatory protein CbpM (cbpM) CSB-YP509216ENU
CSB-EP509216ENU
CSB-BP509216ENU
CSB-MP509216ENU
CSB-EP509216ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Pyrimidine monooxygenase RutA (rutA) CSB-YP509217ENU
CSB-EP509217ENU
CSB-BP509217ENU
CSB-MP509217ENU
CSB-EP509217ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-cysteine desulfhydrase (dcyD) CSB-YP509218ENU
CSB-EP509218ENU
CSB-BP509218ENU
CSB-MP509218ENU
CSB-EP509218ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP509219ENU1
CSB-EP509219ENU1
CSB-BP509219ENU1
CSB-MP509219ENU1
CSB-EP509219ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP509220ENU
CSB-EP509220ENU
CSB-BP509220ENU
CSB-MP509220ENU
CSB-EP509220ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S21 (rpsU) CSB-YP509221ENU
CSB-EP509221ENU
CSB-BP509221ENU
CSB-MP509221ENU
CSB-EP509221ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-tagatose-1,6-bisphosphate aldolase subunit kbaZ (kbaZ) CSB-YP509222ENU
CSB-EP509222ENU
CSB-BP509222ENU
CSB-MP509222ENU
CSB-EP509222ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-ribulose-5-phosphate 3-epimerase UlaE (ulaE) CSB-YP509223ENU
CSB-EP509223ENU
CSB-BP509223ENU
CSB-MP509223ENU
CSB-EP509223ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Iron-sulfur cluster repair protein YtfE (ytfE) CSB-YP509224ENU
CSB-EP509224ENU
CSB-BP509224ENU
CSB-MP509224ENU
CSB-EP509224ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein translocase subunit SecA (secA), partial CSB-YP509225ENU
CSB-EP509225ENU
CSB-BP509225ENU
CSB-MP509225ENU
CSB-EP509225ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamyl-Q tRNA (Asp) synthetase CSB-YP509226ENU
CSB-EP509226ENU
CSB-BP509226ENU
CSB-MP509226ENU
CSB-EP509226ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP509227ENU
CSB-EP509227ENU
CSB-BP509227ENU
CSB-MP509227ENU
CSB-EP509227ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Outer membrane protein assembly factor BamA (bamA) CSB-YP509228ENU
CSB-BP509228ENU
CSB-MP509228ENU
CSB-EP509228ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Proline--tRNA ligase (proS) CSB-YP509229ENU
CSB-EP509229ENU
CSB-BP509229ENU
CSB-MP509229ENU
CSB-EP509229ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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