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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli 50S ribosomal protein L33 (rpmG) CSB-YP509270ENU
CSB-EP509270ENU
CSB-BP509270ENU
CSB-MP509270ENU
CSB-EP509270ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP509271ENU
CSB-EP509271ENU
CSB-BP509271ENU
CSB-MP509271ENU
CSB-EP509271ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC CSB-YP509272ENU
CSB-EP509272ENU
CSB-BP509272ENU
CSB-MP509272ENU
CSB-EP509272ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP509273ENU
CSB-EP509273ENU
CSB-BP509273ENU
CSB-MP509273ENU
CSB-EP509273ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Electron transport complex protein RnfA (rnfA), partial CSB-YP509274ENU1
CSB-EP509274ENU1
CSB-BP509274ENU1
CSB-MP509274ENU1
CSB-EP509274ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transcriptional regulator slyA (slyA) CSB-YP509275ENU
CSB-EP509275ENU
CSB-BP509275ENU
CSB-MP509275ENU
CSB-EP509275ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0061 protein ydiU (ydiU) CSB-YP509276ENU
CSB-EP509276ENU
CSB-BP509276ENU
CSB-MP509276ENU
CSB-EP509276ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP509277ENU
CSB-EP509277ENU
CSB-BP509277ENU
CSB-MP509277ENU
CSB-EP509277ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein RnfH (rnfH) CSB-YP509278ENU
CSB-EP509278ENU
CSB-BP509278ENU
CSB-MP509278ENU
CSB-EP509278ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Carbon storage regulator (csrA) CSB-YP509279ENU
CSB-EP509279ENU
CSB-BP509279ENU
CSB-MP509279ENU
CSB-EP509279ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L34 (rpmH) CSB-YP509280ENU
CSB-EP509280ENU
CSB-BP509280ENU
CSB-MP509280ENU
CSB-EP509280ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Bifunctional protein GlmU (glmU) CSB-YP509281ENU
CSB-EP509281ENU
CSB-BP509281ENU
CSB-MP509281ENU
CSB-EP509281ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein viaA (viaA) CSB-YP509282ENU
CSB-EP509282ENU
CSB-BP509282ENU
CSB-MP509282ENU
CSB-EP509282ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Dihydroxy-acid dehydratase (ilvD) CSB-YP509283ENU
CSB-EP509283ENU
CSB-BP509283ENU
CSB-MP509283ENU
CSB-EP509283ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative ECA polymerase (wzyE), partial CSB-YP509284ENU
CSB-EP509284ENU
CSB-BP509284ENU
CSB-MP509284ENU
CSB-EP509284ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein ves (ves) CSB-YP509285ENU
CSB-EP509285ENU
CSB-BP509285ENU
CSB-MP509285ENU
CSB-EP509285ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0756 membrane protein YeaL (yeaL), partial CSB-YP509286ENU1
CSB-EP509286ENU1
CSB-BP509286ENU1
CSB-MP509286ENU1
CSB-EP509286ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ProP effector (proQ) CSB-YP509287ENU
CSB-EP509287ENU
CSB-BP509287ENU
CSB-MP509287ENU
CSB-EP509287ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP509288ENU
CSB-EP509288ENU
CSB-BP509288ENU
CSB-MP509288ENU
CSB-EP509288ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-fucose isomerase (fucI) CSB-YP509289ENU
CSB-EP509289ENU
CSB-BP509289ENU
CSB-MP509289ENU
CSB-EP509289ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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