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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Copper homeostasis protein CutC (cutC) CSB-YP512572ENU
CSB-EP512572ENU
CSB-BP512572ENU
CSB-MP512572ENU
CSB-EP512572ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Flagellar protein FliT (fliT) CSB-YP512573ENU
CSB-EP512573ENU
CSB-BP512573ENU
CSB-MP512573ENU
CSB-EP512573ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cobalamin synthase (cobS), partial CSB-YP512574ENU1
CSB-EP512574ENU1
CSB-BP512574ENU1
CSB-MP512574ENU1
CSB-EP512574ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP512575ENU
CSB-EP512575ENU
CSB-BP512575ENU
CSB-MP512575ENU
CSB-EP512575ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0597 protein yhaM (yhaM) CSB-YP512576ENU
CSB-EP512576ENU
CSB-BP512576ENU
CSB-MP512576ENU
CSB-EP512576ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional regulator UlaR (ulaR) CSB-YP512577ENU
CSB-EP512577ENU
CSB-BP512577ENU
CSB-MP512577ENU
CSB-EP512577ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP512578ENU
CSB-EP512578ENU
CSB-BP512578ENU
CSB-MP512578ENU
CSB-EP512578ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP512579ENU
CSB-EP512579ENU
CSB-BP512579ENU
CSB-MP512579ENU
CSB-EP512579ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0289 protein yacF (yacF) CSB-YP512580ENU
CSB-EP512580ENU
CSB-BP512580ENU
CSB-MP512580ENU
CSB-EP512580ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Aspartate 1-decarboxylase (panD) CSB-YP512581ENU
CSB-EP512581ENU
CSB-BP512581ENU
CSB-MP512581ENU
CSB-EP512581ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0325 protein yaeH (yaeH) CSB-YP512582ENU
CSB-EP512582ENU
CSB-BP512582ENU
CSB-MP512582ENU
CSB-EP512582ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Lipid-A-disaccharide synthase (lpxB) CSB-YP512583ENU
CSB-EP512583ENU
CSB-BP512583ENU
CSB-MP512583ENU
CSB-EP512583ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Multidrug resistance protein MdtG (mdtG), partial CSB-YP512584ENU
CSB-EP512584ENU
CSB-BP512584ENU
CSB-MP512584ENU
CSB-EP512584ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L32 (rpmF) CSB-YP512585ENU
CSB-EP512585ENU
CSB-BP512585ENU
CSB-MP512585ENU
CSB-EP512585ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Beta-hexosaminidase (nagZ) CSB-YP512586ENU
CSB-EP512586ENU
CSB-BP512586ENU
CSB-MP512586ENU
CSB-EP512586ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable septum site-determining protein MinC (minC) CSB-YP512587ENU
CSB-EP512587ENU
CSB-BP512587ENU
CSB-MP512587ENU
CSB-EP512587ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative multidrug resistance protein MdtD (mdtD), partial CSB-YP512588ENU
CSB-EP512588ENU
CSB-BP512588ENU
CSB-MP512588ENU
CSB-EP512588ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0213 protein yhbQ (yhbQ) CSB-YP512589ENU
CSB-EP512589ENU
CSB-BP512589ENU
CSB-MP512589ENU
CSB-EP512589ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli N-acetylmannosamine kinase (nanK) CSB-YP512590ENU
CSB-EP512590ENU
CSB-BP512590ENU
CSB-MP512590ENU
CSB-EP512590ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Anti-adapter protein iraD (iraD) CSB-YP512591ENU
CSB-EP512591ENU
CSB-BP512591ENU
CSB-MP512591ENU
CSB-EP512591ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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