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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli ATPase ravA (ravA) CSB-YP512632ENU
CSB-EP512632ENU
CSB-BP512632ENU
CSB-MP512632ENU
CSB-EP512632ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 4-alpha-L-fucosyltransferase (wecF) CSB-YP512633ENU
CSB-EP512633ENU
CSB-BP512633ENU
CSB-MP512633ENU
CSB-EP512633ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Succinylglutamate desuccinylase (astE) CSB-YP512634ENU
CSB-EP512634ENU
CSB-BP512634ENU
CSB-MP512634ENU
CSB-EP512634ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP512635ENU
CSB-EP512635ENU
CSB-BP512635ENU
CSB-MP512635ENU
CSB-EP512635ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP512636ENU
CSB-EP512636ENU
CSB-BP512636ENU
CSB-MP512636ENU
CSB-EP512636ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sulfite reductase [NADPH] hemoprotein beta-component (cysI) CSB-YP512637ENU
CSB-EP512637ENU
CSB-BP512637ENU
CSB-MP512637ENU
CSB-EP512637ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-fucose mutarotase (fucU) CSB-YP512638ENU
CSB-EP512638ENU
CSB-BP512638ENU
CSB-MP512638ENU
CSB-EP512638ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Bifunctional protein aas (aas), partial CSB-YP512639ENU1
CSB-EP512639ENU1
CSB-BP512639ENU1
CSB-MP512639ENU1
CSB-EP512639ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0761 membrane protein yihY (yihY), partial CSB-YP512640ENU1
CSB-EP512640ENU1
CSB-BP512640ENU1
CSB-MP512640ENU1
CSB-EP512640ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ferrous-iron efflux pump FieF (fieF), partial CSB-YP512641ENU1
CSB-EP512641ENU1
CSB-BP512641ENU1
CSB-MP512641ENU1
CSB-EP512641ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP512642ENU
CSB-EP512642ENU
CSB-BP512642ENU
CSB-MP512642ENU
CSB-EP512642ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli S-adenosylmethionine synthase (metK) CSB-YP512643ENU
CSB-EP512643ENU
CSB-BP512643ENU
CSB-MP512643ENU
CSB-EP512643ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Membrane-bound lytic murein transglycosylase C (mltC) CSB-YP512644ENU
CSB-EP512644ENU
CSB-BP512644ENU
CSB-MP512644ENU
CSB-EP512644ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamate racemase (murI) CSB-YP512645ENU
CSB-EP512645ENU
CSB-BP512645ENU
CSB-MP512645ENU
CSB-EP512645ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Thiazole synthase (thiG) CSB-YP512646ENU
CSB-EP512646ENU
CSB-BP512646ENU
CSB-MP512646ENU
CSB-EP512646ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein psiE (psiE), partial CSB-YP512647ENU1
CSB-EP512647ENU1
CSB-BP512647ENU1
CSB-MP512647ENU1
CSB-EP512647ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli LexA repressor (lexA) CSB-YP512648ENU
CSB-EP512648ENU
CSB-BP512648ENU
CSB-MP512648ENU
CSB-EP512648ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Elongation factor P (efp) CSB-YP512649ENU
CSB-EP512649ENU
CSB-BP512649ENU
CSB-MP512649ENU
CSB-EP512649ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA mismatch repair protein mutL (mutL) CSB-YP512650ENU
CSB-EP512650ENU
CSB-BP512650ENU
CSB-MP512650ENU
CSB-EP512650ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus gammatolerans Putative thymidine phosphorylase (TGAM_1786), partial CSB-YP512651TMP
CSB-EP512651TMP
CSB-BP512651TMP
CSB-MP512651TMP
CSB-EP512651TMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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