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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Uncharacterized protein yfhE (yfhE) CSB-YP516274BRJ
CSB-EP516274BRJ
CSB-BP516274BRJ
CSB-MP516274BRJ
CSB-EP516274BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfhK (yfhK) CSB-YP516275BRJ
CSB-EP516275BRJ
CSB-BP516275BRJ
CSB-MP516275BRJ
CSB-EP516275BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable A/G-specific adenine glycosylase YfhQ (yfhQ) CSB-YP516276BRJ
CSB-EP516276BRJ
CSB-BP516276BRJ
CSB-MP516276BRJ
CSB-EP516276BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yhbB (yhbB) CSB-YP516277BRJ
CSB-EP516277BRJ
CSB-BP516277BRJ
CSB-MP516277BRJ
CSB-EP516277BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0736 protein yjbA (yjbA) CSB-YP516278BRJ
CSB-EP516278BRJ
CSB-BP516278BRJ
CSB-MP516278BRJ
CSB-EP516278BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative competence protein CoiA (coiA) CSB-YP516279BRJ
CSB-EP516279BRJ
CSB-BP516279BRJ
CSB-MP516279BRJ
CSB-EP516279BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjbK (yjbK) CSB-YP516280BRJ
CSB-EP516280BRJ
CSB-BP516280BRJ
CSB-MP516280BRJ
CSB-EP516280BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Bis (5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] CSB-YP516281BRJ
CSB-EP516281BRJ
CSB-BP516281BRJ
CSB-MP516281BRJ
CSB-EP516281BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sulfur carrier protein ThiS adenylyltransferase (thiF) CSB-YP516282BRJ
CSB-EP516282BRJ
CSB-BP516282BRJ
CSB-MP516282BRJ
CSB-EP516282BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized N-acetyltransferase YjcF (yjcF) CSB-YP516283BRJ
CSB-EP516283BRJ
CSB-BP516283BRJ
CSB-MP516283BRJ
CSB-EP516283BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative ribosomal-protein-alanine acetyltransferase (yjcK) CSB-YP516284BRJ
CSB-EP516284BRJ
CSB-BP516284BRJ
CSB-MP516284BRJ
CSB-EP516284BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjcO (yjcO) CSB-YP516285BRJ
CSB-EP516285BRJ
CSB-BP516285BRJ
CSB-MP516285BRJ
CSB-EP516285BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase yjdA (yjdA) CSB-YP516286BRJ
CSB-EP516286BRJ
CSB-BP516286BRJ
CSB-MP516286BRJ
CSB-EP516286BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjdF (yjdF) CSB-YP516287BRJ
CSB-EP516287BRJ
CSB-BP516287BRJ
CSB-MP516287BRJ
CSB-EP516287BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protoheme IX farnesyltransferase 1 (ctaB1), partial CSB-YP516288BRJ
CSB-EP516288BRJ
CSB-BP516288BRJ
CSB-MP516288BRJ
CSB-EP516288BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protease HtpX homolog (htpX), partial CSB-YP516289BRJ1
CSB-EP516289BRJ1
CSB-BP516289BRJ1
CSB-MP516289BRJ1
CSB-EP516289BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP516290BRJ
CSB-EP516290BRJ
CSB-BP516290BRJ
CSB-MP516290BRJ
CSB-EP516290BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator mhqR (mhqR) CSB-YP516291BRJ
CSB-EP516291BRJ
CSB-BP516291BRJ
CSB-MP516291BRJ
CSB-EP516291BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 7-carboxy-7-deazaguanine synthase (queE) CSB-YP516292BRJ
CSB-EP516292BRJ
CSB-BP516292BRJ
CSB-MP516292BRJ
CSB-EP516292BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ykvR (ykvR) CSB-YP516293BRJ
CSB-EP516293BRJ
CSB-BP516293BRJ
CSB-MP516293BRJ
CSB-EP516293BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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