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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis UPF0714 protein yndL (yndL), partial CSB-YP516314BRJ1
CSB-EP516314BRJ1
CSB-BP516314BRJ1
CSB-MP516314BRJ1
CSB-EP516314BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Aspartyl-phosphate phosphatase ynzD (ynzD) CSB-YP516315BRJ
CSB-EP516315BRJ
CSB-BP516315BRJ
CSB-MP516315BRJ
CSB-EP516315BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Oligoribonuclease nrnB (nrnB) CSB-YP516316BRJ
CSB-EP516316BRJ
CSB-BP516316BRJ
CSB-MP516316BRJ
CSB-EP516316BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yoaQ (yoaQ) CSB-YP516317BRJ
CSB-EP516317BRJ
CSB-BP516317BRJ
CSB-MP516317BRJ
CSB-EP516317BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yobA (yobA) CSB-YP516318BRJ
CSB-EP516318BRJ
CSB-BP516318BRJ
CSB-MP516318BRJ
CSB-EP516318BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yozL (yozL) CSB-YP516319BRJ
CSB-EP516319BRJ
CSB-BP516319BRJ
CSB-MP516319BRJ
CSB-EP516319BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yozO (yozO) CSB-YP516320BRJ
CSB-EP516320BRJ
CSB-BP516320BRJ
CSB-MP516320BRJ
CSB-EP516320BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS (cwlS) CSB-YP516321BRJ
CSB-EP516321BRJ
CSB-BP516321BRJ
CSB-MP516321BRJ
CSB-EP516321BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yojJ (yojJ) CSB-YP516322BRJ
CSB-EP516322BRJ
CSB-BP516322BRJ
CSB-MP516322BRJ
CSB-EP516322BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yojB (yojB), partial CSB-YP516323BRJ1
CSB-EP516323BRJ1
CSB-BP516323BRJ1
CSB-MP516323BRJ1
CSB-EP516323BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yotK (yotK) CSB-YP516324BRJ
CSB-EP516324BRJ
CSB-BP516324BRJ
CSB-MP516324BRJ
CSB-EP516324BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yosU (yosU), partial CSB-YP516325BRJ
CSB-EP516325BRJ
CSB-BP516325BRJ
CSB-MP516325BRJ
CSB-EP516325BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yosH (yosH) CSB-YP516326BRJ
CSB-EP516326BRJ
CSB-BP516326BRJ
CSB-MP516326BRJ
CSB-EP516326BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yosB (yosB) CSB-YP516327BRJ
CSB-EP516327BRJ
CSB-BP516327BRJ
CSB-MP516327BRJ
CSB-EP516327BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yorY (yorY) CSB-YP516328BRJ
CSB-EP516328BRJ
CSB-BP516328BRJ
CSB-MP516328BRJ
CSB-EP516328BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yorR (yorR) CSB-YP516329BRJ
CSB-EP516329BRJ
CSB-BP516329BRJ
CSB-MP516329BRJ
CSB-EP516329BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yorM (yorM) CSB-YP516330BRJ
CSB-EP516330BRJ
CSB-BP516330BRJ
CSB-MP516330BRJ
CSB-EP516330BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yorH (yorH) CSB-YP516331BRJ
CSB-EP516331BRJ
CSB-BP516331BRJ
CSB-MP516331BRJ
CSB-EP516331BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yorC (yorC) CSB-YP516332BRJ
CSB-EP516332BRJ
CSB-BP516332BRJ
CSB-MP516332BRJ
CSB-EP516332BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yoqX (yoqX) CSB-YP516333BRJ
CSB-EP516333BRJ
CSB-BP516333BRJ
CSB-MP516333BRJ
CSB-EP516333BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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