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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Fructosamine deglycase frlB (frlB) CSB-YP516374BRJ
CSB-EP516374BRJ
CSB-BP516374BRJ
CSB-MP516374BRJ
CSB-EP516374BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yurT (yurT) CSB-YP516375BRJ
CSB-EP516375BRJ
CSB-BP516375BRJ
CSB-MP516375BRJ
CSB-EP516375BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Methionine-binding lipoprotein metQ (metQ) CSB-YP516376BRJ
CSB-EP516376BRJ
CSB-BP516376BRJ
CSB-MP516376BRJ
CSB-EP516376BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yusG (yusG) CSB-YP516377BRJ
CSB-EP516377BRJ
CSB-BP516377BRJ
CSB-MP516377BRJ
CSB-EP516377BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable tautomerase yusQ (yusQ) CSB-YP516378BRJ
CSB-EP516378BRJ
CSB-BP516378BRJ
CSB-MP516378BRJ
CSB-EP516378BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable siderophore transport system ATP-binding protein YusV (yusV) CSB-YP516379BRJ
CSB-EP516379BRJ
CSB-BP516379BRJ
CSB-MP516379BRJ
CSB-EP516379BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sensor histidine kinase liaS (liaS), partial CSB-YP516380BRJ
CSB-EP516380BRJ
CSB-BP516380BRJ
CSB-MP516380BRJ
CSB-EP516380BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yvqJ (yvqJ), partial CSB-YP516381BRJ
CSB-EP516381BRJ
CSB-BP516381BRJ
CSB-MP516381BRJ
CSB-EP516381BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative ABC transporter substrate-binding lipoprotein yvgL (yvgL) CSB-YP516382BRJ
CSB-EP516382BRJ
CSB-BP516382BRJ
CSB-MP516382BRJ
CSB-EP516382BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sodium, potassium, lithium and rubidium/H (+) antiporter, partial CSB-YP516383BRJ
CSB-EP516383BRJ
CSB-BP516383BRJ
CSB-MP516383BRJ
CSB-EP516383BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Disulfide bond formation protein C (bdbC), partial CSB-YP516384BRJ1
CSB-EP516384BRJ1
CSB-BP516384BRJ1
CSB-MP516384BRJ1
CSB-EP516384BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis FMN-dependent NADH-azoreductase 2 (azoR2) CSB-YP516385BRJ
CSB-EP516385BRJ
CSB-BP516385BRJ
CSB-MP516385BRJ
CSB-EP516385BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SsrA-binding protein (smpB) CSB-YP516386BRJ
CSB-EP516386BRJ
CSB-BP516386BRJ
CSB-MP516386BRJ
CSB-EP516386BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yvzC (yvzC) CSB-YP516387BRJ
CSB-EP516387BRJ
CSB-BP516387BRJ
CSB-MP516387BRJ
CSB-EP516387BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0056 membrane protein yvbG (yvbG), partial CSB-YP516388BRJ1
CSB-EP516388BRJ1
CSB-BP516388BRJ1
CSB-MP516388BRJ1
CSB-EP516388BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yvbI (yvbI), partial CSB-YP516389BRJ1
CSB-EP516389BRJ1
CSB-BP516389BRJ1
CSB-MP516389BRJ1
CSB-EP516389BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transporter yvbV (yvbV), partial CSB-YP516390BRJ
CSB-EP516390BRJ
CSB-BP516390BRJ
CSB-MP516390BRJ
CSB-EP516390BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yvdS (yvdS), partial CSB-YP516391BRJ1
CSB-EP516391BRJ1
CSB-BP516391BRJ1
CSB-MP516391BRJ1
CSB-EP516391BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative teichuronic acid biosynthesis glycosyltransferase tuaG (tuaG) CSB-YP516392BRJ
CSB-EP516392BRJ
CSB-BP516392BRJ
CSB-MP516392BRJ
CSB-EP516392BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative undecaprenyl-phosphate N-acetylgalactosaminyl 1-phosphate transferase (tuaA), partial CSB-YP516393BRJ1
CSB-EP516393BRJ1
CSB-BP516393BRJ1
CSB-MP516393BRJ1
CSB-EP516393BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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