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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Probable L-serine dehydratase, alpha chain (sdaAA) CSB-YP516514BRJ
CSB-EP516514BRJ
CSB-BP516514BRJ
CSB-MP516514BRJ
CSB-EP516514BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative rRNA methylase ytqB (ytqB) CSB-YP516515BRJ
CSB-EP516515BRJ
CSB-BP516515BRJ
CSB-MP516515BRJ
CSB-EP516515BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator lmrA (lmrA) CSB-YP516516BRJ
CSB-EP516516BRJ
CSB-BP516516BRJ
CSB-MP516516BRJ
CSB-EP516516BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Blue-light photoreceptor (pfyP) CSB-YP516517BRJ
CSB-EP516517BRJ
CSB-BP516517BRJ
CSB-MP516517BRJ
CSB-EP516517BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjlC (yjlC) CSB-YP516518BRJ
CSB-EP516518BRJ
CSB-BP516518BRJ
CSB-MP516518BRJ
CSB-EP516518BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative transcriptional regulator protein yobU (yobU) CSB-YP516519BRJ
CSB-EP516519BRJ
CSB-BP516519BRJ
CSB-MP516519BRJ
CSB-EP516519BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yotB (yotB) CSB-YP516520BRJ
CSB-EP516520BRJ
CSB-BP516520BRJ
CSB-MP516520BRJ
CSB-EP516520BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter permease protein ytlD (ytlD), partial CSB-YP516521BRJ1
CSB-EP516521BRJ1
CSB-BP516521BRJ1
CSB-MP516521BRJ1
CSB-EP516521BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0298 protein ylbG (ylbG) CSB-YP516522BRJ
CSB-EP516522BRJ
CSB-BP516522BRJ
CSB-MP516522BRJ
CSB-EP516522BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter ybcL (ybcL), partial CSB-YP516523BRJ
CSB-EP516523BRJ
CSB-BP516523BRJ
CSB-MP516523BRJ
CSB-EP516523BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable glutamine ABC transporter permease protein glnM (glnM), partial CSB-YP516524BRJ1
CSB-EP516524BRJ1
CSB-BP516524BRJ1
CSB-MP516524BRJ1
CSB-EP516524BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glutamine transport ATP-binding protein GlnQ (glnQ) CSB-YP516525BRJ
CSB-EP516525BRJ
CSB-BP516525BRJ
CSB-MP516525BRJ
CSB-EP516525BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ylbB (ylbB) CSB-YP516526BRJ
CSB-EP516526BRJ
CSB-BP516526BRJ
CSB-MP516526BRJ
CSB-EP516526BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 50S ribosomal protein L32 (rpmF) CSB-YP516527BRJ
CSB-EP516527BRJ
CSB-BP516527BRJ
CSB-MP516527BRJ
CSB-EP516527BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yloA (yloA), partial CSB-YP516528BRJ
CSB-EP516528BRJ
CSB-BP516528BRJ
CSB-MP516528BRJ
CSB-EP516528BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yotJ (yotJ) CSB-YP516529BRJ
CSB-EP516529BRJ
CSB-BP516529BRJ
CSB-MP516529BRJ
CSB-EP516529BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yxnB (yxnB) CSB-YP516530BRJ
CSB-EP516530BRJ
CSB-BP516530BRJ
CSB-MP516530BRJ
CSB-EP516530BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative HTH-type transcriptional regulator yvbF (yvbF) CSB-YP516531BRJ
CSB-EP516531BRJ
CSB-BP516531BRJ
CSB-MP516531BRJ
CSB-EP516531BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable 2,4-dienoyl-CoA reductase (fadH) CSB-YP516532BRJ
CSB-EP516532BRJ
CSB-BP516532BRJ
CSB-MP516532BRJ
CSB-EP516532BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Transcriptional regulatory protein desR (desR) CSB-YP516533BRJ
CSB-EP516533BRJ
CSB-BP516533BRJ
CSB-MP516533BRJ
CSB-EP516533BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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