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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yopY (yopY) CSB-YP516534BRJ
CSB-EP516534BRJ
CSB-BP516534BRJ
CSB-MP516534BRJ
CSB-EP516534BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative HMP/thiamine permease protein ykoE (ykoE), partial CSB-YP516535BRJ1
CSB-EP516535BRJ1
CSB-BP516535BRJ1
CSB-MP516535BRJ1
CSB-EP516535BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ylbA (ylbA) CSB-YP516536BRJ
CSB-EP516536BRJ
CSB-BP516536BRJ
CSB-MP516536BRJ
CSB-EP516536BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable ATP-dependent DNA helicase recQ (recQ), partial CSB-YP516537BRJ
CSB-EP516537BRJ
CSB-BP516537BRJ
CSB-MP516537BRJ
CSB-EP516537BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sensor histidine kinase desK (desK), partial CSB-YP516538BRJ1
CSB-EP516538BRJ1
CSB-BP516538BRJ1
CSB-MP516538BRJ1
CSB-EP516538BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfmA (yfmA) CSB-YP516539BRJ
CSB-EP516539BRJ
CSB-BP516539BRJ
CSB-MP516539BRJ
CSB-EP516539BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized HTH-type transcriptional regulator yopS (yopS) CSB-YP516540BRJ
CSB-EP516540BRJ
CSB-BP516540BRJ
CSB-MP516540BRJ
CSB-EP516540BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yobJ (yobJ), partial CSB-YP516541BRJ1
CSB-EP516541BRJ1
CSB-BP516541BRJ1
CSB-MP516541BRJ1
CSB-EP516541BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydjB (ydjB) CSB-YP516542BRJ
CSB-EP516542BRJ
CSB-BP516542BRJ
CSB-MP516542BRJ
CSB-EP516542BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yobI (yobI), partial CSB-YP516543BRJ
CSB-EP516543BRJ
CSB-BP516543BRJ
CSB-MP516543BRJ
CSB-EP516543BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP516544BRJ
CSB-EP516544BRJ
CSB-BP516544BRJ
CSB-MP516544BRJ
CSB-EP516544BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phosphopantetheine adenylyltransferase (coaD) CSB-YP516545BRJ
CSB-EP516545BRJ
CSB-BP516545BRJ
CSB-MP516545BRJ
CSB-EP516545BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter substrate-binding lipoprotein yvrC (yvrC) CSB-YP516546BRJ
CSB-EP516546BRJ
CSB-BP516546BRJ
CSB-MP516546BRJ
CSB-EP516546BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0756 membrane protein YtwI (ytwI), partial CSB-YP516547BRJ1
CSB-EP516547BRJ1
CSB-BP516547BRJ1
CSB-MP516547BRJ1
CSB-EP516547BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative L,D-transpeptidase YkuD (ykuD) CSB-YP516548BRJ
CSB-EP516548BRJ
CSB-BP516548BRJ
CSB-MP516548BRJ
CSB-EP516548BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ykuM (ykuM) CSB-YP516549BRJ
CSB-EP516549BRJ
CSB-BP516549BRJ
CSB-MP516549BRJ
CSB-EP516549BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Fe (3+)-citrate import system permease protein yfmE, partial CSB-YP516550BRJ
CSB-EP516550BRJ
CSB-BP516550BRJ
CSB-MP516550BRJ
CSB-EP516550BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative cation exchanger yfkE (yfkE), partial CSB-YP516551BRJ
CSB-EP516551BRJ
CSB-BP516551BRJ
CSB-MP516551BRJ
CSB-EP516551BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis RNA polymerase sigma factor sigO (sigO) CSB-YP516552BRJ
CSB-EP516552BRJ
CSB-BP516552BRJ
CSB-MP516552BRJ
CSB-EP516552BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yotN (yotN) CSB-YP516553BRJ
CSB-EP516553BRJ
CSB-BP516553BRJ
CSB-MP516553BRJ
CSB-EP516553BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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