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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis UvrABC system protein A (uvrA), partial CSB-YP516554BRJ
CSB-EP516554BRJ
CSB-BP516554BRJ
CSB-MP516554BRJ
CSB-EP516554BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative carboxypeptidase yodJ (yodJ) CSB-YP516555BRJ
CSB-EP516555BRJ
CSB-BP516555BRJ
CSB-MP516555BRJ
CSB-EP516555BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Hydroxymethylglutaryl-CoA lyase yngG (yngG) CSB-YP516556BRJ
CSB-EP516556BRJ
CSB-BP516556BRJ
CSB-MP516556BRJ
CSB-EP516556BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ykuO (ykuO) CSB-YP516557BRJ
CSB-EP516557BRJ
CSB-BP516557BRJ
CSB-MP516557BRJ
CSB-EP516557BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Type-2 restriction enzyme BsuMI component ydiS (ydiS) CSB-YP516558BRJ
CSB-EP516558BRJ
CSB-BP516558BRJ
CSB-MP516558BRJ
CSB-EP516558BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative enoyl-CoA hydratase/isomerase yngF (yngF) CSB-YP516559BRJ
CSB-EP516559BRJ
CSB-BP516559BRJ
CSB-MP516559BRJ
CSB-EP516559BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable ABC transporter permease ytrC (ytrC), partial CSB-YP516560BRJ
CSB-EP516560BRJ
CSB-BP516560BRJ
CSB-MP516560BRJ
CSB-EP516560BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yobQ (yobQ) CSB-YP516561BRJ
CSB-EP516561BRJ
CSB-BP516561BRJ
CSB-MP516561BRJ
CSB-EP516561BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yflI (yflI), partial CSB-YP516562BRJ
CSB-EP516562BRJ
CSB-BP516562BRJ
CSB-MP516562BRJ
CSB-EP516562BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative HMP/thiamine-binding protein ykoF (ykoF) CSB-YP516563BRJ
CSB-EP516563BRJ
CSB-BP516563BRJ
CSB-MP516563BRJ
CSB-EP516563BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ytfI (ytfI) CSB-YP516564BRJ
CSB-EP516564BRJ
CSB-BP516564BRJ
CSB-MP516564BRJ
CSB-EP516564BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ytpI (ytpI), partial CSB-YP516565BRJ1
CSB-EP516565BRJ1
CSB-BP516565BRJ1
CSB-MP516565BRJ1
CSB-EP516565BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yczE (yczE), partial CSB-YP516566BRJ1
CSB-EP516566BRJ1
CSB-BP516566BRJ1
CSB-MP516566BRJ1
CSB-EP516566BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Fe (3+)-citrate import system permease protein yfmD, partial CSB-YP516567BRJ
CSB-EP516567BRJ
CSB-BP516567BRJ
CSB-MP516567BRJ
CSB-EP516567BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable BsuMI modification methylase subunit ydiO (ydiO) CSB-YP516568BRJ
CSB-EP516568BRJ
CSB-BP516568BRJ
CSB-MP516568BRJ
CSB-EP516568BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable zinc transport system ATP-binding protein AdcC (adcC) CSB-YP516569BRJ
CSB-EP516569BRJ
CSB-BP516569BRJ
CSB-MP516569BRJ
CSB-EP516569BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Lipoteichoic acid synthase 2 (ltaS2), partial CSB-YP516570BRJ1
CSB-EP516570BRJ1
CSB-BP516570BRJ1
CSB-MP516570BRJ1
CSB-EP516570BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized symporter yjmB (yjmB), partial CSB-YP516571BRJ
CSB-EP516571BRJ
CSB-BP516571BRJ
CSB-MP516571BRJ
CSB-EP516571BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative lipoprotein yerB (yerB) CSB-YP516572BRJ
CSB-EP516572BRJ
CSB-BP516572BRJ
CSB-MP516572BRJ
CSB-EP516572BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative monooxygenase moxC (moxC) CSB-YP516573BRJ
CSB-EP516573BRJ
CSB-BP516573BRJ
CSB-MP516573BRJ
CSB-EP516573BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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