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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis UPF0176 protein ybfQ (ybfQ) CSB-YP518236BRJ
CSB-EP518236BRJ
CSB-BP518236BRJ
CSB-MP518236BRJ
CSB-EP518236BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Branched-chain-amino-acid transaminase 1 (ilvE) CSB-YP518237BRJ
CSB-EP518237BRJ
CSB-BP518237BRJ
CSB-MP518237BRJ
CSB-EP518237BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Tryptophan RNA-binding attenuator protein inhibitory protein (rtpA) CSB-YP518238BRJ
CSB-EP518238BRJ
CSB-BP518238BRJ
CSB-MP518238BRJ
CSB-EP518238BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized methyltransferase ycgJ (ycgJ) CSB-YP518239BRJ
CSB-EP518239BRJ
CSB-BP518239BRJ
CSB-MP518239BRJ
CSB-EP518239BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yczJ (yczJ) CSB-YP518240BRJ
CSB-EP518240BRJ
CSB-BP518240BRJ
CSB-MP518240BRJ
CSB-EP518240BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydcI (ydcI), partial CSB-YP518241BRJ
CSB-EP518241BRJ
CSB-BP518241BRJ
CSB-MP518241BRJ
CSB-EP518241BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydzF (ydzF) CSB-YP518242BRJ
CSB-EP518242BRJ
CSB-BP518242BRJ
CSB-MP518242BRJ
CSB-EP518242BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Swarming motility protein SwrC (swrC), partial CSB-YP518243BRJ
CSB-EP518243BRJ
CSB-BP518243BRJ
CSB-MP518243BRJ
CSB-EP518243BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0720 protein yeeF (yeeF), partial CSB-YP518244BRJ
CSB-EP518244BRJ
CSB-BP518244BRJ
CSB-MP518244BRJ
CSB-EP518244BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase yesF (yesF) CSB-YP518245BRJ
CSB-EP518245BRJ
CSB-BP518245BRJ
CSB-MP518245BRJ
CSB-EP518245BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sensor histidine kinase yesM (yesM), partial CSB-YP518246BRJ
CSB-EP518246BRJ
CSB-BP518246BRJ
CSB-MP518246BRJ
CSB-EP518246BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Unsaturated rhamnogalacturonyl hydrolase YesR (yesR) CSB-YP518247BRJ
CSB-EP518247BRJ
CSB-BP518247BRJ
CSB-MP518247BRJ
CSB-EP518247BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Rhamnogalacturonan endolyase yesW (yesW), partial CSB-YP518248BRJ
CSB-EP518248BRJ
CSB-BP518248BRJ
CSB-MP518248BRJ
CSB-EP518248BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0702 transmembrane protein yetF (yetF), partial CSB-YP518249BRJ1
CSB-EP518249BRJ1
CSB-BP518249BRJ1
CSB-MP518249BRJ1
CSB-EP518249BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein YetJ (yetJ), partial CSB-YP518250BRJ1
CSB-EP518250BRJ1
CSB-BP518250BRJ1
CSB-MP518250BRJ1
CSB-EP518250BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative DNA-3-methyladenine glycosylase yfjP (yfjP) CSB-YP518251BRJ
CSB-EP518251BRJ
CSB-BP518251BRJ
CSB-MP518251BRJ
CSB-EP518251BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfjM (yfjM) CSB-YP518252BRJ
CSB-EP518252BRJ
CSB-BP518252BRJ
CSB-MP518252BRJ
CSB-EP518252BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfjE (yfjE), partial CSB-YP518253BRJ1
CSB-EP518253BRJ1
CSB-BP518253BRJ1
CSB-MP518253BRJ1
CSB-EP518253BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative membrane-bound acyltransferase YfiQ (yfiQ), partial CSB-YP518254BRJ
CSB-EP518254BRJ
CSB-BP518254BRJ
CSB-MP518254BRJ
CSB-EP518254BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yfiV (yfiV) CSB-YP518255BRJ
CSB-EP518255BRJ
CSB-BP518255BRJ
CSB-MP518255BRJ
CSB-EP518255BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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