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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Putative NAD (P)H nitroreductase yfhC CSB-YP518256BRJ
CSB-EP518256BRJ
CSB-BP518256BRJ
CSB-MP518256BRJ
CSB-EP518256BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Epimerase family protein yfhF (yfhF) CSB-YP518257BRJ
CSB-EP518257BRJ
CSB-BP518257BRJ
CSB-MP518257BRJ
CSB-EP518257BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfhJ (yfhJ) CSB-YP518258BRJ
CSB-EP518258BRJ
CSB-BP518258BRJ
CSB-MP518258BRJ
CSB-EP518258BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfhP (yfhP), partial CSB-YP518259BRJ1
CSB-EP518259BRJ1
CSB-BP518259BRJ1
CSB-MP518259BRJ1
CSB-EP518259BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative tRNA (cytidine (34)-2'-O)-methyltransferase CSB-YP518260BRJ
CSB-EP518260BRJ
CSB-BP518260BRJ
CSB-MP518260BRJ
CSB-EP518260BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjaZ (yjaZ) CSB-YP518261BRJ
CSB-EP518261BRJ
CSB-BP518261BRJ
CSB-MP518261BRJ
CSB-EP518261BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yjbE (yjbE), partial CSB-YP518262BRJ1
CSB-EP518262BRJ1
CSB-BP518262BRJ1
CSB-MP518262BRJ1
CSB-EP518262BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative murein lytic transglycosylase yjbJ (yjbJ) CSB-YP518263BRJ
CSB-EP518263BRJ
CSB-BP518263BRJ
CSB-MP518263BRJ
CSB-EP518263BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable inorganic polyphosphate/ATP-NAD kinase 1 (ppnK1) CSB-YP518264BRJ
CSB-EP518264BRJ
CSB-BP518264BRJ
CSB-MP518264BRJ
CSB-EP518264BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Thiazole synthase (thiG) CSB-YP518265BRJ
CSB-EP518265BRJ
CSB-BP518265BRJ
CSB-MP518265BRJ
CSB-EP518265BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sporulation-specific transcription factor spoVIF (spoVIF) CSB-YP518266BRJ
CSB-EP518266BRJ
CSB-BP518266BRJ
CSB-MP518266BRJ
CSB-EP518266BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yjcL (yjcL), partial CSB-YP518267BRJ
CSB-EP518267BRJ
CSB-BP518267BRJ
CSB-MP518267BRJ
CSB-EP518267BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjcR (yjcR) CSB-YP518268BRJ
CSB-EP518268BRJ
CSB-BP518268BRJ
CSB-MP518268BRJ
CSB-EP518268BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis PTS system mannose-specific EIIBCA component (manP), partial CSB-YP518269BRJ
CSB-EP518269BRJ
CSB-BP518269BRJ
CSB-MP518269BRJ
CSB-EP518269BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjdH (yjdH) CSB-YP518270BRJ
CSB-EP518270BRJ
CSB-BP518270BRJ
CSB-MP518270BRJ
CSB-EP518270BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis RNA polymerase sigma factor sigI (sigI) CSB-YP518271BRJ
CSB-EP518271BRJ
CSB-BP518271BRJ
CSB-MP518271BRJ
CSB-EP518271BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ykzE (ykzE) CSB-YP518272BRJ
CSB-EP518272BRJ
CSB-BP518272BRJ
CSB-MP518272BRJ
CSB-EP518272BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Methylthioribose kinase (mtnK) CSB-YP518273BRJ
CSB-EP518273BRJ
CSB-BP518273BRJ
CSB-MP518273BRJ
CSB-EP518273BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP518274BRJ
CSB-EP518274BRJ
CSB-BP518274BRJ
CSB-MP518274BRJ
CSB-EP518274BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 7-cyano-7-deazaguanine synthase (queC) CSB-YP518275BRJ
CSB-EP518275BRJ
CSB-BP518275BRJ
CSB-MP518275BRJ
CSB-EP518275BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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