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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Agrobacterium vitis Uncharacterized protein in TAR-II ttuC' 3'region CSB-YP518456AYT
CSB-EP518456AYT
CSB-BP518456AYT
CSB-MP518456AYT
CSB-EP518456AYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative oxidoreductase ytkK (ytkK) CSB-YP518457BRJ
CSB-EP518457BRJ
CSB-BP518457BRJ
CSB-MP518457BRJ
CSB-EP518457BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter ATP-binding protein YtlC (ytlC) CSB-YP518458BRJ
CSB-EP518458BRJ
CSB-BP518458BRJ
CSB-MP518458BRJ
CSB-EP518458BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized glycosyltransferase ykcC (ykcC), partial CSB-YP518459BRJ1
CSB-EP518459BRJ1
CSB-BP518459BRJ1
CSB-MP518459BRJ1
CSB-EP518459BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ytrP (ytrP), partial CSB-YP518460BRJ
CSB-EP518460BRJ
CSB-BP518460BRJ
CSB-MP518460BRJ
CSB-EP518460BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0720 protein yobL (yobL), partial CSB-YP518461BRJ
CSB-EP518461BRJ
CSB-BP518461BRJ
CSB-MP518461BRJ
CSB-EP518461BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Manganese transport system ATP-binding protein MntB (mntB) CSB-YP518462BRJ
CSB-EP518462BRJ
CSB-BP518462BRJ
CSB-MP518462BRJ
CSB-EP518462BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yebC (yebC), partial CSB-YP518463BRJ1
CSB-EP518463BRJ1
CSB-BP518463BRJ1
CSB-MP518463BRJ1
CSB-EP518463BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized N-acetyltransferase YtmI (ytmI) CSB-YP518464BRJ
CSB-EP518464BRJ
CSB-BP518464BRJ
CSB-MP518464BRJ
CSB-EP518464BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Altronate oxidoreductase (uxaB) CSB-YP518465BRJ
CSB-EP518465BRJ
CSB-BP518465BRJ
CSB-MP518465BRJ
CSB-EP518465BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized SPBc2 prophage-derived protein yoqJ (yoqJ) CSB-YP518466BRJ
CSB-EP518466BRJ
CSB-BP518466BRJ
CSB-MP518466BRJ
CSB-EP518466BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative flagellin yvzB (yvzB) CSB-YP518467BRJ
CSB-EP518467BRJ
CSB-BP518467BRJ
CSB-MP518467BRJ
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative cytochrome P450 YjiB (yjiB) CSB-YP518468BRJ
CSB-EP518468BRJ
CSB-BP518468BRJ
CSB-MP518468BRJ
CSB-EP518468BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ybcI (ybcI) CSB-YP518469BRJ
CSB-EP518469BRJ
CSB-BP518469BRJ
CSB-MP518469BRJ
CSB-EP518469BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Manganese-binding lipoprotein mntA (mntA) CSB-YP518470BRJ
CSB-EP518470BRJ
CSB-BP518470BRJ
CSB-MP518470BRJ
CSB-EP518470BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable ATP-dependent DNA ligase ykoU (ykoU), partial CSB-YP518471BRJ
CSB-EP518471BRJ
CSB-BP518471BRJ
CSB-MP518471BRJ
CSB-EP518471BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP518472BRJ
CSB-EP518472BRJ
CSB-BP518472BRJ
CSB-MP518472BRJ
CSB-EP518472BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Histidinol-phosphatase (hisK) CSB-YP518473BRJ
CSB-EP518473BRJ
CSB-BP518473BRJ
CSB-MP518473BRJ
CSB-EP518473BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfkI (yfkI) CSB-YP518474BRJ
CSB-EP518474BRJ
CSB-BP518474BRJ
CSB-MP518474BRJ
CSB-EP518474BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glyceraldehyde-3-phosphate dehydrogenase 2 (gapB) CSB-YP518475BRJ
CSB-EP518475BRJ
CSB-BP518475BRJ
CSB-MP518475BRJ
CSB-EP518475BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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