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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis UPF0721 transmembrane protein ytnM (ytnM), partial CSB-YP518476BRJ
CSB-EP518476BRJ
CSB-BP518476BRJ
CSB-MP518476BRJ
CSB-EP518476BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative ribonuclease-like protein yfkH (yfkH), partial CSB-YP518477BRJ1
CSB-EP518477BRJ1
CSB-BP518477BRJ1
CSB-MP518477BRJ1
CSB-EP518477BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter permease protein yvrB (yvrB), partial CSB-YP518478BRJ
CSB-EP518478BRJ
CSB-BP518478BRJ
CSB-MP518478BRJ
CSB-EP518478BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Molybdenum cofactor biosynthesis protein B (moaB) CSB-YP518479BRJ
CSB-EP518479BRJ
CSB-BP518479BRJ
CSB-MP518479BRJ
CSB-EP518479BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sporulation membrane protein ytrI (ytrI), partial CSB-YP518480BRJ1
CSB-EP518480BRJ1
CSB-BP518480BRJ1
CSB-MP518480BRJ1
CSB-EP518480BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ylbL (ylbL), partial CSB-YP518481BRJ
CSB-EP518481BRJ
CSB-BP518481BRJ
CSB-MP518481BRJ
CSB-EP518481BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable cysteine synthase (ytkP) CSB-YP518482BRJ
CSB-EP518482BRJ
CSB-BP518482BRJ
CSB-MP518482BRJ
CSB-EP518482BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HPr kinase/phosphorylase (hprK) CSB-YP518483BRJ
CSB-EP518483BRJ
CSB-BP518483BRJ
CSB-MP518483BRJ
CSB-EP518483BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0354 protein ytpQ (ytpQ) CSB-YP518484BRJ
CSB-EP518484BRJ
CSB-BP518484BRJ
CSB-MP518484BRJ
CSB-EP518484BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein YkgB (ykgB) CSB-YP518485BRJ
CSB-EP518485BRJ
CSB-BP518485BRJ
CSB-MP518485BRJ
CSB-EP518485BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ythB (ythB), partial CSB-YP518486BRJ
CSB-EP518486BRJ
CSB-BP518486BRJ
CSB-MP518486BRJ
CSB-EP518486BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Fe (3+)-citrate import ATP-binding protein YfmF CSB-YP518487BRJ
CSB-EP518487BRJ
CSB-BP518487BRJ
CSB-MP518487BRJ
CSB-EP518487BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis o-succinylbenzoate synthase (menC) CSB-YP518488BRJ
CSB-EP518488BRJ
CSB-BP518488BRJ
CSB-MP518488BRJ
CSB-EP518488BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis tRNA (guanine-N (7)-)-methyltransferase CSB-YP518489BRJ
CSB-EP518489BRJ
CSB-BP518489BRJ
CSB-MP518489BRJ
CSB-EP518489BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized AAA domain-containing protein YrvN (yrvN) CSB-YP518490BRJ
CSB-EP518490BRJ
CSB-BP518490BRJ
CSB-MP518490BRJ
CSB-EP518490BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yoaT (yoaT), partial CSB-YP518491BRJ
CSB-EP518491BRJ
CSB-BP518491BRJ
CSB-MP518491BRJ
CSB-EP518491BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yosV (yosV) CSB-YP518492BRJ
CSB-EP518492BRJ
CSB-BP518492BRJ
CSB-MP518492BRJ
CSB-EP518492BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative ring-cleaving dioxygenase mhqE (mhqE) CSB-YP518493BRJ
CSB-EP518493BRJ
CSB-BP518493BRJ
CSB-MP518493BRJ
CSB-EP518493BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ykoI (ykoI), partial CSB-YP518494BRJ
CSB-EP518494BRJ
CSB-BP518494BRJ
CSB-MP518494BRJ
CSB-EP518494BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Thioredoxin-like protein ykuU (ykuU) CSB-YP518495BRJ
CSB-EP518495BRJ
CSB-BP518495BRJ
CSB-MP518495BRJ
CSB-EP518495BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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