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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP520078BRJ
CSB-EP520078BRJ
CSB-BP520078BRJ
CSB-MP520078BRJ
CSB-EP520078BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SpoIVD-associated factor A (safA) CSB-YP520079BRJ
CSB-EP520079BRJ
CSB-BP520079BRJ
CSB-MP520079BRJ
CSB-EP520079BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ytdP (ytdP), partial CSB-YP520080BRJ1
CSB-EP520080BRJ1
CSB-BP520080BRJ1
CSB-MP520080BRJ1
CSB-EP520080BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yuaE (yuaE) CSB-YP520081BRJ
CSB-EP520081BRJ
CSB-BP520081BRJ
CSB-MP520081BRJ
CSB-EP520081BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yubD (yubD), partial CSB-YP520082BRJ
CSB-EP520082BRJ
CSB-BP520082BRJ
CSB-MP520082BRJ
CSB-EP520082BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yuzE (yuzE) CSB-YP520083BRJ
CSB-EP520083BRJ
CSB-BP520083BRJ
CSB-MP520083BRJ
CSB-EP520083BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Bacteriophage SPP1 adsorption protein yueB (yueB), partial CSB-YP520084BRJ
CSB-EP520084BRJ
CSB-BP520084BRJ
CSB-MP520084BRJ
CSB-EP520084BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yuiD (yuiD), partial CSB-YP520085BRJ1
CSB-EP520085BRJ1
CSB-BP520085BRJ1
CSB-MP520085BRJ1
CSB-EP520085BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein YutM (yutM) CSB-YP520086BRJ
CSB-EP520086BRJ
CSB-BP520086BRJ
CSB-MP520086BRJ
CSB-EP520086BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative nitrogen fixation protein yutI (yutI) CSB-YP520087BRJ
CSB-EP520087BRJ
CSB-BP520087BRJ
CSB-MP520087BRJ
CSB-EP520087BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yutD (yutD) CSB-YP520088BRJ
CSB-EP520088BRJ
CSB-BP520088BRJ
CSB-MP520088BRJ
CSB-EP520088BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yunC (yunC) CSB-YP520089BRJ
CSB-EP520089BRJ
CSB-BP520089BRJ
CSB-MP520089BRJ
CSB-EP520089BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Purine catabolism regulatory protein (pucR), partial CSB-YP520090BRJ
CSB-EP520090BRJ
CSB-BP520090BRJ
CSB-MP520090BRJ
CSB-EP520090BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable xanthine dehydrogenase subunit E (pucE) CSB-YP520091BRJ
CSB-EP520091BRJ
CSB-BP520091BRJ
CSB-MP520091BRJ
CSB-EP520091BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Purine catabolism protein PucG (pucG) CSB-YP520092BRJ
CSB-EP520092BRJ
CSB-BP520092BRJ
CSB-MP520092BRJ
CSB-EP520092BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Fructosamine kinase FrlD (frlD) CSB-YP520093BRJ
CSB-EP520093BRJ
CSB-BP520093BRJ
CSB-MP520093BRJ
CSB-EP520093BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter extracellular-binding protein yurO (yurO) CSB-YP520094BRJ
CSB-EP520094BRJ
CSB-BP520094BRJ
CSB-MP520094BRJ
CSB-EP520094BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis FeS cluster assembly protein sufB (sufB) CSB-YP520095BRJ
CSB-EP520095BRJ
CSB-BP520095BRJ
CSB-MP520095BRJ
CSB-EP520095BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yurZ (yurZ) CSB-YP520096BRJ
CSB-EP520096BRJ
CSB-BP520096BRJ
CSB-MP520096BRJ
CSB-EP520096BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yusD (yusD) CSB-YP520097BRJ
CSB-EP520097BRJ
CSB-BP520097BRJ
CSB-MP520097BRJ
CSB-EP520097BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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