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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Uncharacterized protein ytvB (ytvB), partial CSB-YP520278BRJ1
CSB-EP520278BRJ1
CSB-BP520278BRJ1
CSB-MP520278BRJ1
CSB-EP520278BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sporulation-delaying protein sdpA (sdpA) CSB-YP520279BRJ
CSB-EP520279BRJ
CSB-BP520279BRJ
CSB-MP520279BRJ
CSB-EP520279BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized N-acetyltransferase YodP (yodP) CSB-YP520280BRJ
CSB-EP520280BRJ
CSB-BP520280BRJ
CSB-MP520280BRJ
CSB-EP520280BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis L-cystine import ATP-binding protein TcyN (tcyN) CSB-YP520281BRJ
CSB-EP520281BRJ
CSB-BP520281BRJ
CSB-MP520281BRJ
CSB-EP520281BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0361 protein yoaM (yoaM) CSB-YP520282BRJ
CSB-EP520282BRJ
CSB-BP520282BRJ
CSB-MP520282BRJ
CSB-EP520282BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Histidine biosynthesis bifunctional protein HisIE (hisI) CSB-YP520283BRJ
CSB-EP520283BRJ
CSB-BP520283BRJ
CSB-MP520283BRJ
CSB-EP520283BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived deoxyuridine 5'-triphosphate nucleotidohydrolase yosS (yosS) CSB-YP520284BRJ
CSB-EP520284BRJ
CSB-BP520284BRJ
CSB-MP520284BRJ
CSB-EP520284BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative aminopeptidase ytoP (ytoP) CSB-YP520285BRJ
CSB-EP520285BRJ
CSB-BP520285BRJ
CSB-MP520285BRJ
CSB-EP520285BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yfkF (yfkF), partial CSB-YP520286BRJ
CSB-EP520286BRJ
CSB-BP520286BRJ
CSB-MP520286BRJ
CSB-EP520286BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable inorganic polyphosphate/ATP-NAD kinase 2 (ppnK2) CSB-YP520287BRJ
CSB-EP520287BRJ
CSB-BP520287BRJ
CSB-MP520287BRJ
CSB-EP520287BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative tRNA-binding protein ytpR (ytpR) CSB-YP520288BRJ
CSB-EP520288BRJ
CSB-BP520288BRJ
CSB-MP520288BRJ
CSB-EP520288BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ykoM (ykoM) CSB-YP520289BRJ
CSB-EP520289BRJ
CSB-BP520289BRJ
CSB-MP520289BRJ
CSB-EP520289BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yeeD (yeeD) CSB-YP520290BRJ
CSB-EP520290BRJ
CSB-BP520290BRJ
CSB-MP520290BRJ
CSB-EP520290BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 50S ribosomal protein L31 type B (rpmE2) CSB-YP520291BRJ
CSB-EP520291BRJ
CSB-BP520291BRJ
CSB-MP520291BRJ
CSB-EP520291BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative hydrolase YtaP (ytaP) CSB-YP520292BRJ
CSB-EP520292BRJ
CSB-BP520292BRJ
CSB-MP520292BRJ
CSB-EP520292BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter ATP-binding protein YvrO (yvrO) CSB-YP520293BRJ
CSB-EP520293BRJ
CSB-BP520293BRJ
CSB-MP520293BRJ
CSB-EP520293BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yoaO (yoaO) CSB-YP520294BRJ
CSB-EP520294BRJ
CSB-BP520294BRJ
CSB-MP520294BRJ
CSB-EP520294BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Formyltetrahydrofolate deformylase (purU) CSB-YP520295BRJ
CSB-EP520295BRJ
CSB-BP520295BRJ
CSB-MP520295BRJ
CSB-EP520295BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis DNA polymerase I (polA), partial CSB-YP520296BRJ
CSB-EP520296BRJ
CSB-BP520296BRJ
CSB-MP520296BRJ
CSB-EP520296BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Carboxy-terminal processing protease CtpB (ctpB) CSB-YP520297BRJ
CSB-EP520297BRJ
CSB-BP520297BRJ
CSB-MP520297BRJ
CSB-EP520297BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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