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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Uncharacterized protein yccF (yccF) CSB-YP520218BRJ
CSB-EP520218BRJ
CSB-BP520218BRJ
CSB-MP520218BRJ
CSB-EP520218BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yfkQ (yfkQ), partial CSB-YP520219BRJ1
CSB-EP520219BRJ1
CSB-BP520219BRJ1
CSB-MP520219BRJ1
CSB-EP520219BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yopT (yopT) CSB-YP520220BRJ
CSB-EP520220BRJ
CSB-BP520220BRJ
CSB-MP520220BRJ
CSB-EP520220BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yceB (yceB) CSB-YP520221BRJ
CSB-EP520221BRJ
CSB-BP520221BRJ
CSB-MP520221BRJ
CSB-EP520221BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yopZ (yopZ), partial CSB-YP520222BRJ1
CSB-EP520222BRJ1
CSB-BP520222BRJ1
CSB-MP520222BRJ1
CSB-EP520222BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yocB (yocB) CSB-YP520223BRJ
CSB-EP520223BRJ
CSB-BP520223BRJ
CSB-MP520223BRJ
CSB-EP520223BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized sodium-dependent transporter yocS (yocS), partial CSB-YP520224BRJ
CSB-EP520224BRJ
CSB-BP520224BRJ
CSB-MP520224BRJ
CSB-EP520224BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Transcriptional regulatory protein CitT (citT) CSB-YP520225BRJ
CSB-EP520225BRJ
CSB-BP520225BRJ
CSB-MP520225BRJ
CSB-EP520225BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized UDP-glucosyltransferase yjiC (yjiC) CSB-YP520226BRJ
CSB-EP520226BRJ
CSB-BP520226BRJ
CSB-MP520226BRJ
CSB-EP520226BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Branched-chain amino acid transport system carrier protein BraB (braB), partial CSB-YP520227BRJ
CSB-EP520227BRJ
CSB-BP520227BRJ
CSB-MP520227BRJ
CSB-EP520227BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjfA (yjfA) CSB-YP520228BRJ
CSB-EP520228BRJ
CSB-BP520228BRJ
CSB-MP520228BRJ
CSB-EP520228BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Unsaturated rhamnogalacturonyl hydrolase YteR (yteR) CSB-YP520229BRJ
CSB-EP520229BRJ
CSB-BP520229BRJ
CSB-MP520229BRJ
CSB-EP520229BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative spore germination protein YfkT (yfkT), partial CSB-YP520230BRJ
CSB-EP520230BRJ
CSB-BP520230BRJ
CSB-MP520230BRJ
CSB-EP520230BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ATP-dependent DNA helicase pcrA (pcrA), partial CSB-YP520231BRJ
CSB-EP520231BRJ
CSB-BP520231BRJ
CSB-MP520231BRJ
CSB-EP520231BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ykuJ (ykuJ) CSB-YP520232BRJ
CSB-EP520232BRJ
CSB-BP520232BRJ
CSB-MP520232BRJ
CSB-EP520232BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cytochrome c-551 (cccB) CSB-YP520233BRJ
CSB-EP520233BRJ
CSB-BP520233BRJ
CSB-MP520233BRJ
CSB-EP520233BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yobK (yobK) CSB-YP520234BRJ
CSB-EP520234BRJ
CSB-BP520234BRJ
CSB-MP520234BRJ
CSB-EP520234BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative membrane protein insertion efficiency factor (ytjA) CSB-YP520235BRJ
CSB-EP520235BRJ
CSB-BP520235BRJ
CSB-MP520235BRJ
CSB-EP520235BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydiN (ydiN) CSB-YP520236BRJ
CSB-EP520236BRJ
CSB-BP520236BRJ
CSB-MP520236BRJ
CSB-EP520236BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sporulation-delaying protein sdpB (sdpB), partial CSB-YP520237BRJ1
CSB-EP520237BRJ1
CSB-BP520237BRJ1
CSB-MP520237BRJ1
CSB-EP520237BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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