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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Killing factor sdpC (sdpC) CSB-YP520198BRJ
CSB-EP520198BRJ
CSB-BP520198BRJ
CSB-MP520198BRJ
CSB-EP520198BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Mannonate dehydratase (uxuA) CSB-YP520199BRJ
CSB-EP520199BRJ
CSB-BP520199BRJ
CSB-MP520199BRJ
CSB-EP520199BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yvmC (yvmC) CSB-YP520200BRJ
CSB-EP520200BRJ
CSB-BP520200BRJ
CSB-MP520200BRJ
CSB-EP520200BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative amidohydrolase ytcJ (ytcJ), partial CSB-YP520201BRJ
CSB-EP520201BRJ
CSB-BP520201BRJ
CSB-MP520201BRJ
CSB-EP520201BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable oligo-1,6-glucosidase 2 (ycdG), partial CSB-YP520202BRJ
CSB-EP520202BRJ
CSB-BP520202BRJ
CSB-MP520202BRJ
CSB-EP520202BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yteU (yteU), partial CSB-YP520203BRJ1
CSB-EP520203BRJ1
CSB-BP520203BRJ1
CSB-MP520203BRJ1
CSB-EP520203BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein YceE (yceE) CSB-YP520204BRJ
CSB-EP520204BRJ
CSB-BP520204BRJ
CSB-MP520204BRJ
CSB-EP520204BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjjA (yjjA) CSB-YP520205BRJ
CSB-EP520205BRJ
CSB-BP520205BRJ
CSB-MP520205BRJ
CSB-EP520205BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yrrD (yrrD) CSB-YP520206BRJ
CSB-EP520206BRJ
CSB-BP520206BRJ
CSB-MP520206BRJ
CSB-EP520206BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yotD (yotD) CSB-YP520207BRJ
CSB-EP520207BRJ
CSB-BP520207BRJ
CSB-MP520207BRJ
CSB-EP520207BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yodN (yodN) CSB-YP520208BRJ
CSB-EP520208BRJ
CSB-BP520208BRJ
CSB-MP520208BRJ
CSB-EP520208BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein YjqC (yjqC) CSB-YP520209BRJ
CSB-EP520209BRJ
CSB-BP520209BRJ
CSB-MP520209BRJ
CSB-EP520209BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjlA (yjlA), partial CSB-YP520210BRJ
CSB-EP520210BRJ
CSB-BP520210BRJ
CSB-MP520210BRJ
CSB-EP520210BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydjG (ydjG), partial CSB-YP520211BRJ1
CSB-EP520211BRJ1
CSB-BP520211BRJ1
CSB-MP520211BRJ1
CSB-EP520211BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0065 protein yflP (yflP) CSB-YP520212BRJ
CSB-EP520212BRJ
CSB-BP520212BRJ
CSB-MP520212BRJ
CSB-EP520212BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Magnesium transporter mgtE (mgtE), partial CSB-YP520213BRJ1
CSB-EP520213BRJ1
CSB-BP520213BRJ1
CSB-MP520213BRJ1
CSB-EP520213BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived putative antirepressor protein yoqD (yoqD) CSB-YP520214BRJ
CSB-EP520214BRJ
CSB-BP520214BRJ
CSB-MP520214BRJ
CSB-EP520214BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Nitric oxide synthase oxygenase (nos) CSB-YP520215BRJ
CSB-EP520215BRJ
CSB-BP520215BRJ
CSB-MP520215BRJ
CSB-EP520215BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable coenzyme A transferase subunit beta (yodR) CSB-YP520216BRJ
CSB-EP520216BRJ
CSB-BP520216BRJ
CSB-MP520216BRJ
CSB-EP520216BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0118 membrane protein yrrI (yrrI), partial CSB-YP520217BRJ
CSB-EP520217BRJ
CSB-BP520217BRJ
CSB-MP520217BRJ
CSB-EP520217BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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