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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP522000BRJ1
CSB-EP522000BRJ1
CSB-BP522000BRJ1
CSB-MP522000BRJ1
CSB-EP522000BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0755 protein yrrL (yrrL) CSB-YP522001BRJ
CSB-EP522001BRJ
CSB-BP522001BRJ
CSB-MP522001BRJ
CSB-EP522001BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Stress response protein ykoL (ykoL) CSB-YP522002BRJ
CSB-EP522002BRJ
CSB-BP522002BRJ
CSB-MP522002BRJ
CSB-EP522002BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized sugar kinase YdjE (ydjE) CSB-YP522003BRJ
CSB-EP522003BRJ
CSB-BP522003BRJ
CSB-MP522003BRJ
CSB-EP522003BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ykuK (ykuK) CSB-YP522004BRJ
CSB-EP522004BRJ
CSB-BP522004BRJ
CSB-MP522004BRJ
CSB-EP522004BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase yvrD (yvrD) CSB-YP522005BRJ
CSB-EP522005BRJ
CSB-BP522005BRJ
CSB-MP522005BRJ
CSB-EP522005BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis (R,R)-butanediol dehydrogenase CSB-YP522006BRJ
CSB-EP522006BRJ
CSB-BP522006BRJ
CSB-MP522006BRJ
CSB-EP522006BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ybcH (ybcH) CSB-YP522007BRJ
CSB-EP522007BRJ
CSB-BP522007BRJ
CSB-MP522007BRJ
CSB-EP522007BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yeaA (yeaA) CSB-YP522008BRJ
CSB-EP522008BRJ
CSB-BP522008BRJ
CSB-MP522008BRJ
CSB-EP522008BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative NADP-dependent oxidoreductase yfmJ (yfmJ) CSB-YP522009BRJ
CSB-EP522009BRJ
CSB-BP522009BRJ
CSB-MP522009BRJ
CSB-EP522009BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional repressor yvoA (yvoA) CSB-YP522010BRJ
CSB-EP522010BRJ
CSB-BP522010BRJ
CSB-MP522010BRJ
CSB-EP522010BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phosphoglucosamine mutase (glmM) CSB-YP522011BRJ
CSB-EP522011BRJ
CSB-BP522011BRJ
CSB-MP522011BRJ
CSB-EP522011BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yoqB (yoqB) CSB-YP522012BRJ
CSB-EP522012BRJ
CSB-BP522012BRJ
CSB-MP522012BRJ
CSB-EP522012BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Transcription factor fapR (fapR) CSB-YP522013BRJ
CSB-EP522013BRJ
CSB-BP522013BRJ
CSB-MP522013BRJ
CSB-EP522013BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Magnesium-chelatase 60 kDa subunit (bchD), partial CSB-YP522014RLF
CSB-EP522014RLF
CSB-BP522014RLF
CSB-MP522014RLF
CSB-EP522014RLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable murein peptide carboxypeptidase (ykfA) CSB-YP522015BRJ
CSB-EP522015BRJ
CSB-BP522015BRJ
CSB-MP522015BRJ
CSB-EP522015BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Repressor rok (rok) CSB-YP522016BRJ
CSB-EP522016BRJ
CSB-BP522016BRJ
CSB-MP522016BRJ
CSB-EP522016BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative UDP-glucose 6-dehydrogenase ytcA (ytcA) CSB-YP522017BRJ
CSB-EP522017BRJ
CSB-BP522017BRJ
CSB-MP522017BRJ
CSB-EP522017BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized metallophosphoesterase ykuE (ykuE) CSB-YP522018BRJ
CSB-EP522018BRJ
CSB-BP522018BRJ
CSB-MP522018BRJ
CSB-EP522018BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Polyketide biosynthesis acyltransferase homolog PksD (pksD) CSB-YP522019BRJ
CSB-EP522019BRJ
CSB-BP522019BRJ
CSB-MP522019BRJ
CSB-EP522019BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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