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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Uncharacterized protein ykgA (ykgA) CSB-YP521960BRJ
CSB-EP521960BRJ
CSB-BP521960BRJ
CSB-MP521960BRJ
CSB-EP521960BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yvkA (yvkA), partial CSB-YP521961BRJ
CSB-EP521961BRJ
CSB-BP521961BRJ
CSB-MP521961BRJ
CSB-EP521961BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Serine/threonine-protein kinase PrkC (prkC), partial CSB-YP521962BRJ
CSB-EP521962BRJ
CSB-BP521962BRJ
CSB-MP521962BRJ
CSB-EP521962BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative ABC transporter permease protein AmyC (amyC), partial CSB-YP521963BRJ1
CSB-EP521963BRJ1
CSB-BP521963BRJ1
CSB-MP521963BRJ1
CSB-EP521963BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Alanine--tRNA ligase (alaS), partial CSB-YP521964BRJ
CSB-EP521964BRJ
CSB-BP521964BRJ
CSB-MP521964BRJ
CSB-EP521964BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ytcD (ytcD) CSB-YP521965BRJ
CSB-EP521965BRJ
CSB-BP521965BRJ
CSB-MP521965BRJ
CSB-EP521965BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized lipoprotein ycdA (ycdA) CSB-YP521966BRJ
CSB-EP521966BRJ
CSB-BP521966BRJ
CSB-MP521966BRJ
CSB-EP521966BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Biotin carboxylase 2 (accC2) CSB-YP521967BRJ
CSB-EP521967BRJ
CSB-BP521967BRJ
CSB-MP521967BRJ
CSB-EP521967BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein ytsP (ytsP) CSB-YP521968BRJ
CSB-EP521968BRJ
CSB-BP521968BRJ
CSB-MP521968BRJ
CSB-EP521968BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized amino acid permease YecA (yecA), partial CSB-YP521969BRJ
CSB-EP521969BRJ
CSB-BP521969BRJ
CSB-MP521969BRJ
CSB-EP521969BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative HMP/thiamine permease protein YkoC (ykoC), partial CSB-YP521970BRJ1
CSB-EP521970BRJ1
CSB-BP521970BRJ1
CSB-MP521970BRJ1
CSB-EP521970BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0721 transmembrane protein yjnA (yjnA), partial CSB-YP521971BRJ1
CSB-EP521971BRJ1
CSB-BP521971BRJ1
CSB-MP521971BRJ1
CSB-EP521971BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjzC (yjzC) CSB-YP521972BRJ
CSB-EP521972BRJ
CSB-BP521972BRJ
CSB-MP521972BRJ
CSB-EP521972BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis AB hydrolase superfamily protein ydjP (ydjP) CSB-YP521973BRJ
CSB-EP521973BRJ
CSB-BP521973BRJ
CSB-MP521973BRJ
CSB-EP521973BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable tRNA sulfurtransferase (thiI) CSB-YP521974BRJ
CSB-EP521974BRJ
CSB-BP521974BRJ
CSB-MP521974BRJ
CSB-EP521974BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Bifunctional oligoribonuclease and PAP phosphatase nrnA (nrnA) CSB-YP521975BRJ
CSB-EP521975BRJ
CSB-BP521975BRJ
CSB-MP521975BRJ
CSB-EP521975BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yopM (yopM) CSB-YP521976BRJ
CSB-EP521976BRJ
CSB-BP521976BRJ
CSB-MP521976BRJ
CSB-EP521976BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yoaR (yoaR) CSB-YP521977BRJ
CSB-EP521977BRJ
CSB-BP521977BRJ
CSB-MP521977BRJ
CSB-EP521977BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized amino acid permease YtnA (ytnA), partial CSB-YP521978BRJ
CSB-EP521978BRJ
CSB-BP521978BRJ
CSB-MP521978BRJ
CSB-EP521978BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfmB (yfmB) CSB-YP521979BRJ
CSB-EP521979BRJ
CSB-BP521979BRJ
CSB-MP521979BRJ
CSB-EP521979BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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