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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Putative phosphohydrolase yueE (yueE) CSB-YP523365BRJ
CSB-EP523365BRJ
CSB-BP523365BRJ
CSB-MP523365BRJ
CSB-EP523365BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative biotin transporter BioYB (bioYB), partial CSB-YP523366BRJ1
CSB-EP523366BRJ1
CSB-BP523366BRJ1
CSB-MP523366BRJ1
CSB-EP523366BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yuiC (yuiC) CSB-YP523367BRJ
CSB-EP523367BRJ
CSB-BP523367BRJ
CSB-MP523367BRJ
CSB-EP523367BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Diaminopimelate epimerase (dapF) CSB-YP523368BRJ
CSB-EP523368BRJ
CSB-BP523368BRJ
CSB-MP523368BRJ
CSB-EP523368BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative disulfide oxidoreductase yuzD (yuzD) CSB-YP523369BRJ
CSB-EP523369BRJ
CSB-BP523369BRJ
CSB-MP523369BRJ
CSB-EP523369BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0331 protein yutE (yutE) CSB-YP523370BRJ
CSB-EP523370BRJ
CSB-BP523370BRJ
CSB-MP523370BRJ
CSB-EP523370BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sporulation protein yunB (yunB), partial CSB-YP523371BRJ1
CSB-EP523371BRJ1
CSB-BP523371BRJ1
CSB-MP523371BRJ1
CSB-EP523371BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yunG (yunG) CSB-YP523372BRJ
CSB-EP523372BRJ
CSB-BP523372BRJ
CSB-MP523372BRJ
CSB-EP523372BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uric acid degradation bifunctional protein PucL (pucL) CSB-YP523373BRJ
CSB-BP523373BRJ
CSB-MP523373BRJ
CSB-EP523373BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable xanthine dehydrogenase subunit C (pucC) CSB-YP523374BRJ
CSB-EP523374BRJ
CSB-BP523374BRJ
CSB-MP523374BRJ
CSB-EP523374BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Allantoate amidohydrolase (pucF) CSB-YP523375BRJ
CSB-EP523375BRJ
CSB-BP523375BRJ
CSB-MP523375BRJ
CSB-EP523375BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yurQ (yurQ) CSB-YP523376BRJ
CSB-EP523376BRJ
CSB-BP523376BRJ
CSB-MP523376BRJ
CSB-EP523376BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable cysteine desulfurase (csd) CSB-YP523377BRJ
CSB-EP523377BRJ
CSB-BP523377BRJ
CSB-MP523377BRJ
CSB-EP523377BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Methionine import ATP-binding protein MetN (metN) CSB-YP523378BRJ
CSB-EP523378BRJ
CSB-BP523378BRJ
CSB-MP523378BRJ
CSB-EP523378BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Short-chain dehydrogenase/reductase homolog yusS (yusS) CSB-YP523379BRJ
CSB-EP523379BRJ
CSB-BP523379BRJ
CSB-MP523379BRJ
CSB-EP523379BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yusW (yusW) CSB-YP523380BRJ
CSB-EP523380BRJ
CSB-BP523380BRJ
CSB-MP523380BRJ
CSB-EP523380BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein liaF (liaF), partial CSB-YP523381BRJ1
CSB-EP523381BRJ1
CSB-BP523381BRJ1
CSB-MP523381BRJ1
CSB-EP523381BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative arginine/ornithine antiporter (yvsH), partial CSB-YP523382BRJ
CSB-EP523382BRJ
CSB-BP523382BRJ
CSB-MP523382BRJ
CSB-EP523382BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative molybdenum transport system permease protein yvgM (yvgM), partial CSB-YP523383BRJ1
CSB-EP523383BRJ1
CSB-BP523383BRJ1
CSB-MP523383BRJ1
CSB-EP523383BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ), partial CSB-YP523384BRJ
CSB-EP523384BRJ
CSB-BP523384BRJ
CSB-MP523384BRJ
CSB-EP523384BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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