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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis SPBc2 prophage-derived protein BhlB (bhlB), partial CSB-YP523345BRJ1
CSB-EP523345BRJ1
CSB-BP523345BRJ1
CSB-MP523345BRJ1
CSB-EP523345BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yolC (yolC) CSB-YP523346BRJ
CSB-EP523346BRJ
CSB-BP523346BRJ
CSB-MP523346BRJ
CSB-EP523346BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yokJ (yokJ) CSB-YP523347BRJ
CSB-EP523347BRJ
CSB-BP523347BRJ
CSB-MP523347BRJ
CSB-EP523347BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yokE (yokE) CSB-YP523348BRJ
CSB-EP523348BRJ
CSB-BP523348BRJ
CSB-MP523348BRJ
CSB-EP523348BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ypzA (ypzA) CSB-YP523349BRJ
CSB-EP523349BRJ
CSB-BP523349BRJ
CSB-MP523349BRJ
CSB-EP523349BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqzF (yqzF), partial CSB-YP523350BRJ1
CSB-EP523350BRJ1
CSB-BP523350BRJ1
CSB-MP523350BRJ1
CSB-EP523350BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phosphatase rapE inhibitor (phrE) CSB-YP523351BRJ
CSB-EP523351BRJ
CSB-BP523351BRJ
CSB-MP523351BRJ
CSB-EP523351BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized methyltransferase YrrT (yrrT) CSB-YP523352BRJ
CSB-EP523352BRJ
CSB-BP523352BRJ
CSB-MP523352BRJ
CSB-EP523352BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protease yrrN (yrrN) CSB-YP523353BRJ
CSB-EP523353BRJ
CSB-BP523353BRJ
CSB-MP523353BRJ
CSB-EP523353BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yrzK (yrzK) CSB-YP523354BRJ
CSB-EP523354BRJ
CSB-BP523354BRJ
CSB-MP523354BRJ
CSB-EP523354BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yrvC (yrvC) CSB-YP523355BRJ
CSB-EP523355BRJ
CSB-BP523355BRJ
CSB-MP523355BRJ
CSB-EP523355BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0092 membrane protein yrbF (yrbF), partial CSB-YP523356BRJ
CSB-EP523356BRJ
CSB-BP523356BRJ
CSB-MP523356BRJ
CSB-EP523356BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative serine/threonine-protein kinase yrzF (yrzF) CSB-YP523357BRJ
CSB-EP523357BRJ
CSB-BP523357BRJ
CSB-MP523357BRJ
CSB-EP523357BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ytzH (ytzH) CSB-YP523358BRJ
CSB-EP523358BRJ
CSB-BP523358BRJ
CSB-MP523358BRJ
CSB-EP523358BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized RNA pseudouridine synthase YtzG (ytzG) CSB-YP523359BRJ
CSB-EP523359BRJ
CSB-BP523359BRJ
CSB-MP523359BRJ
CSB-EP523359BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized N-acetyltransferase YuaI (yuaI) CSB-YP523360BRJ
CSB-EP523360BRJ
CSB-BP523360BRJ
CSB-MP523360BRJ
CSB-EP523360BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Ktr system potassium uptake protein A (ktrA) CSB-YP523361BRJ
CSB-EP523361BRJ
CSB-BP523361BRJ
CSB-MP523361BRJ
CSB-EP523361BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Exo-glucosaminidase lytG (lytG) CSB-YP523362BRJ
CSB-EP523362BRJ
CSB-BP523362BRJ
CSB-MP523362BRJ
CSB-EP523362BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yuzA (yuzA), partial CSB-YP523363BRJ1
CSB-EP523363BRJ1
CSB-BP523363BRJ1
CSB-MP523363BRJ1
CSB-EP523363BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yueH (yueH) CSB-YP523364BRJ
CSB-EP523364BRJ
CSB-BP523364BRJ
CSB-MP523364BRJ
CSB-EP523364BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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