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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Protein phosphatase prpC (prpC) CSB-YP523546BRJ
CSB-EP523546BRJ
CSB-BP523546BRJ
CSB-MP523546BRJ
CSB-EP523546BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0714 protein yjqB (yjqB) CSB-YP523547BRJ
CSB-EP523547BRJ
CSB-BP523547BRJ
CSB-MP523547BRJ
CSB-EP523547BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized HTH-type transcriptional regulator yopO (yopO) CSB-YP523548BRJ
CSB-EP523548BRJ
CSB-BP523548BRJ
CSB-MP523548BRJ
CSB-EP523548BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjeA (yjeA), partial CSB-YP523549BRJ
CSB-EP523549BRJ
CSB-BP523549BRJ
CSB-MP523549BRJ
CSB-EP523549BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized spore protein ytfJ (ytfJ) CSB-YP523550BRJ
CSB-EP523550BRJ
CSB-BP523550BRJ
CSB-MP523550BRJ
CSB-EP523550BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Precorrin-2 dehydrogenase (sirC) CSB-YP523551BRJ
CSB-EP523551BRJ
CSB-BP523551BRJ
CSB-MP523551BRJ
CSB-EP523551BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Pectin lyase (pelB) CSB-YP523552BRJ
CSB-EP523552BRJ
CSB-BP523552BRJ
CSB-MP523552BRJ
CSB-EP523552BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Polyketide biosynthesis malonyl CoA-acyl carrier protein transacylase pksC (pksC) CSB-YP523553BRJ
CSB-EP523553BRJ
CSB-BP523553BRJ
CSB-MP523553BRJ
CSB-EP523553BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator msmR (msmR) CSB-YP523554BRJ
CSB-EP523554BRJ
CSB-BP523554BRJ
CSB-MP523554BRJ
CSB-EP523554BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yoqC (yoqC) CSB-YP523555BRJ
CSB-EP523555BRJ
CSB-BP523555BRJ
CSB-MP523555BRJ
CSB-EP523555BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative hydrolase MhqD (mhqD) CSB-YP523556BRJ
CSB-EP523556BRJ
CSB-BP523556BRJ
CSB-MP523556BRJ
CSB-EP523556BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP523557BRJ
CSB-EP523557BRJ
CSB-BP523557BRJ
CSB-MP523557BRJ
CSB-EP523557BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis L-cystine-binding protein tcyK (tcyK) CSB-YP523558BRJ
CSB-EP523558BRJ
CSB-BP523558BRJ
CSB-MP523558BRJ
CSB-EP523558BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable DNA repair protein ykoV (ykoV) CSB-YP523559BRJ
CSB-EP523559BRJ
CSB-BP523559BRJ
CSB-MP523559BRJ
CSB-EP523559BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative transporter yoaB (yoaB), partial CSB-YP523560BRJ
CSB-EP523560BRJ
CSB-BP523560BRJ
CSB-MP523560BRJ
CSB-EP523560BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative carbonic anhydrase YtiB (ytiB) CSB-YP523561BRJ
CSB-EP523561BRJ
CSB-BP523561BRJ
CSB-MP523561BRJ
CSB-EP523561BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycine betaine/carnitine/choline transport system permease protein OpuCB (opuCB), partial CSB-YP523562BRJ1
CSB-EP523562BRJ1
CSB-BP523562BRJ1
CSB-MP523562BRJ1
CSB-EP523562BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable tautomerase yolI (yolI) CSB-YP523563BRJ
CSB-EP523563BRJ
CSB-BP523563BRJ
CSB-MP523563BRJ
CSB-EP523563BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yflB (yflB) CSB-YP523564BRJ
CSB-EP523564BRJ
CSB-BP523564BRJ
CSB-MP523564BRJ
CSB-EP523564BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Septation ring formation regulator EzrA (ezrA), partial CSB-YP523565BRJ
CSB-EP523565BRJ
CSB-BP523565BRJ
CSB-MP523565BRJ
CSB-EP523565BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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