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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Uncharacterized protein ytzD (ytzD) CSB-YP523505BRJ
CSB-EP523505BRJ
CSB-BP523505BRJ
CSB-MP523505BRJ
CSB-EP523505BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis L-Ala-D/L-Glu epimerase (ykfB) CSB-YP523506BRJ
CSB-EP523506BRJ
CSB-BP523506BRJ
CSB-MP523506BRJ
CSB-EP523506BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0348 protein ylbM (ylbM) CSB-YP523507BRJ
CSB-EP523507BRJ
CSB-BP523507BRJ
CSB-MP523507BRJ
CSB-EP523507BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis PTS system N-acetylglucosamine-specific EIICB component (nagP), partial CSB-YP523508BRJ
CSB-EP523508BRJ
CSB-BP523508BRJ
CSB-MP523508BRJ
CSB-EP523508BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator CymR (cymR) CSB-YP523509BRJ
CSB-EP523509BRJ
CSB-BP523509BRJ
CSB-MP523509BRJ
CSB-EP523509BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized N-acetyltransferase YfmK (yfmK) CSB-YP523510BRJ
CSB-EP523510BRJ
CSB-BP523510BRJ
CSB-MP523510BRJ
CSB-EP523510BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yflK (yflK) CSB-YP523511BRJ
CSB-EP523511BRJ
CSB-BP523511BRJ
CSB-MP523511BRJ
CSB-EP523511BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yttB (yttB), partial CSB-YP523512BRJ
CSB-EP523512BRJ
CSB-BP523512BRJ
CSB-MP523512BRJ
CSB-EP523512BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yoeD (yoeD) CSB-YP523513BRJ
CSB-EP523513BRJ
CSB-BP523513BRJ
CSB-MP523513BRJ
CSB-EP523513BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP523514BRJ
CSB-EP523514BRJ
CSB-BP523514BRJ
CSB-MP523514BRJ
CSB-EP523514BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yefB (yefB) CSB-YP523515BRJ
CSB-EP523515BRJ
CSB-BP523515BRJ
CSB-MP523515BRJ
CSB-EP523515BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfkD (yfkD) CSB-YP523516BRJ
CSB-EP523516BRJ
CSB-BP523516BRJ
CSB-MP523516BRJ
CSB-EP523516BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ylbC (ylbC), partial CSB-YP523517BRJ
CSB-EP523517BRJ
CSB-BP523517BRJ
CSB-MP523517BRJ
CSB-EP523517BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjqA (yjqA) CSB-YP523518BRJ
CSB-EP523518BRJ
CSB-BP523518BRJ
CSB-MP523518BRJ
CSB-EP523518BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable glutamine ABC transporter permease protein glnP (glnP), partial CSB-YP523519BRJ1
CSB-EP523519BRJ1
CSB-BP523519BRJ1
CSB-MP523519BRJ1
CSB-EP523519BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjlB (yjlB) CSB-YP523520BRJ
CSB-EP523520BRJ
CSB-BP523520BRJ
CSB-MP523520BRJ
CSB-EP523520BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ycdB (ycdB) CSB-YP523521BRJ
CSB-EP523521BRJ
CSB-BP523521BRJ
CSB-MP523521BRJ
CSB-EP523521BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yvlB (yvlB) CSB-YP523522BRJ
CSB-EP523522BRJ
CSB-BP523522BRJ
CSB-MP523522BRJ
CSB-EP523522BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative Holliday junction resolvase (yrrK) CSB-YP523523BRJ
CSB-EP523523BRJ
CSB-BP523523BRJ
CSB-MP523523BRJ
CSB-EP523523BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative glutaredoxin ytnI (ytnI) CSB-YP523524BRJ
CSB-EP523524BRJ
CSB-BP523524BRJ
CSB-MP523524BRJ
CSB-EP523524BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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