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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Sirohydrochlorin ferrochelatase (sirB) CSB-YP521980BRJ
CSB-EP521980BRJ
CSB-BP521980BRJ
CSB-MP521980BRJ
CSB-EP521980BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sensor histidine kinase ykoH (ykoH), partial CSB-YP521981BRJ
CSB-EP521981BRJ
CSB-BP521981BRJ
CSB-MP521981BRJ
CSB-EP521981BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yvkB (yvkB) CSB-YP521982BRJ
CSB-EP521982BRJ
CSB-BP521982BRJ
CSB-MP521982BRJ
CSB-EP521982BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Histidinol dehydrogenase (hisD) CSB-YP521983BRJ
CSB-EP521983BRJ
CSB-BP521983BRJ
CSB-MP521983BRJ
CSB-EP521983BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis YbbR-like domain-containing protein ybbR (ybbR) CSB-YP521984BRJ
CSB-EP521984BRJ
CSB-BP521984BRJ
CSB-MP521984BRJ
CSB-EP521984BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Thiamine pyrophosphokinase (thiN) CSB-YP521985BRJ
CSB-EP521985BRJ
CSB-BP521985BRJ
CSB-MP521985BRJ
CSB-EP521985BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydiM (ydiM) CSB-YP521986BRJ
CSB-EP521986BRJ
CSB-BP521986BRJ
CSB-MP521986BRJ
CSB-EP521986BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase YtbE (ytbE) CSB-YP521987BRJ
CSB-EP521987BRJ
CSB-BP521987BRJ
CSB-MP521987BRJ
CSB-EP521987BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP521988BRJ
CSB-EP521988BRJ
CSB-BP521988BRJ
CSB-MP521988BRJ
CSB-EP521988BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0753 protein ybcC (ybcC), partial CSB-YP521989BRJ
CSB-EP521989BRJ
CSB-BP521989BRJ
CSB-MP521989BRJ
CSB-EP521989BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ytaB (ytaB), partial CSB-YP521990BRJ1
CSB-EP521990BRJ1
CSB-BP521990BRJ1
CSB-MP521990BRJ1
CSB-EP521990BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yoaU (yoaU) CSB-YP521991BRJ
CSB-EP521991BRJ
CSB-BP521991BRJ
CSB-MP521991BRJ
CSB-EP521991BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ytpB (ytpB) CSB-YP521992BRJ
CSB-EP521992BRJ
CSB-BP521992BRJ
CSB-MP521992BRJ
CSB-EP521992BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yjzD (yjzD), partial CSB-YP521993BRJ1
CSB-EP521993BRJ1
CSB-BP521993BRJ1
CSB-MP521993BRJ1
CSB-EP521993BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yvlC (yvlC), partial CSB-YP521994BRJ1
CSB-EP521994BRJ1
CSB-BP521994BRJ1
CSB-MP521994BRJ1
CSB-EP521994BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yceJ (yceJ), partial CSB-YP521995BRJ
CSB-EP521995BRJ
CSB-BP521995BRJ
CSB-MP521995BRJ
CSB-EP521995BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ydjJ (ydjJ), partial CSB-YP521996BRJ1
CSB-EP521996BRJ1
CSB-BP521996BRJ1
CSB-MP521996BRJ1
CSB-EP521996BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable flavodoxin-1 (ykuN) CSB-YP521997BRJ
CSB-EP521997BRJ
CSB-BP521997BRJ
CSB-MP521997BRJ
CSB-EP521997BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uroporphyrinogen-III C-methyltransferase (sumT) CSB-YP521998BRJ
CSB-EP521998BRJ
CSB-BP521998BRJ
CSB-MP521998BRJ
CSB-EP521998BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yloV (yloV), partial CSB-YP521999BRJ
CSB-EP521999BRJ
CSB-BP521999BRJ
CSB-MP521999BRJ
CSB-EP521999BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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