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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Bacitracin export ATP-binding protein BceA (bceA) CSB-YP518516BRJ
CSB-EP518516BRJ
CSB-BP518516BRJ
CSB-MP518516BRJ
CSB-EP518516BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ATPase YjoB (yjoB) CSB-YP518517BRJ
CSB-EP518517BRJ
CSB-BP518517BRJ
CSB-MP518517BRJ
CSB-EP518517BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative ABC transporter permease protein AmyD (amyD), partial CSB-YP518518BRJ1
CSB-EP518518BRJ1
CSB-BP518518BRJ1
CSB-MP518518BRJ1
CSB-EP518518BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Oxalate decarboxylase oxdC (oxdC) CSB-YP518519BRJ
CSB-EP518519BRJ
CSB-BP518519BRJ
CSB-MP518519BRJ
CSB-EP518519BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfmG (yfmG) CSB-YP518520BRJ
CSB-EP518520BRJ
CSB-BP518520BRJ
CSB-MP518520BRJ
CSB-EP518520BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP518521BRJ
CSB-EP518521BRJ
CSB-BP518521BRJ
CSB-MP518521BRJ
CSB-EP518521BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase yjmC (yjmC) CSB-YP518522BRJ
CSB-EP518522BRJ
CSB-BP518522BRJ
CSB-MP518522BRJ
CSB-EP518522BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycine betaine/carnitine/choline transport system permease protein OpuCD (opuCD), partial CSB-YP518523BRJ1
CSB-EP518523BRJ1
CSB-BP518523BRJ1
CSB-MP518523BRJ1
CSB-EP518523BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 1 (fabHA) CSB-YP518524BRJ
CSB-EP518524BRJ
CSB-BP518524BRJ
CSB-MP518524BRJ
CSB-EP518524BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized glycosyltransferase ykoT (ykoT), partial CSB-YP518525BRJ1
CSB-EP518525BRJ1
CSB-BP518525BRJ1
CSB-MP518525BRJ1
CSB-EP518525BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydjO (ydjO) CSB-YP518526BRJ
CSB-EP518526BRJ
CSB-BP518526BRJ
CSB-MP518526BRJ
CSB-EP518526BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sulfate adenylyltransferase (sat) CSB-YP518527BRJ
CSB-EP518527BRJ
CSB-BP518527BRJ
CSB-MP518527BRJ
CSB-EP518527BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable polyketide biosynthesis zinc-dependent hydrolase pksB (pksB) CSB-YP518528BRJ
CSB-EP518528BRJ
CSB-BP518528BRJ
CSB-MP518528BRJ
CSB-EP518528BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Organic hydroperoxide resistance transcriptional regulator (ohrR) CSB-YP518529BRJ
CSB-EP518529BRJ
CSB-BP518529BRJ
CSB-MP518529BRJ
CSB-EP518529BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0180 protein ykuS (ykuS) CSB-YP518530BRJ
CSB-EP518530BRJ
CSB-BP518530BRJ
CSB-MP518530BRJ
CSB-EP518530BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydjI (ydjI) CSB-YP518531BRJ
CSB-EP518531BRJ
CSB-BP518531BRJ
CSB-MP518531BRJ
CSB-EP518531BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized phosphotransferase yvkC (yvkC), partial CSB-YP518532BRJ
CSB-EP518532BRJ
CSB-BP518532BRJ
CSB-MP518532BRJ
CSB-EP518532BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Stage II sporulation protein SB (spoIISB) CSB-YP518533BRJ
CSB-EP518533BRJ
CSB-BP518533BRJ
CSB-MP518533BRJ
CSB-EP518533BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uronate isomerase (uxaC) CSB-YP518534BRJ
CSB-EP518534BRJ
CSB-BP518534BRJ
CSB-MP518534BRJ
CSB-EP518534BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cell division ATP-binding protein FtsE (ftsE) CSB-YP518535BRJ
CSB-EP518535BRJ
CSB-BP518535BRJ
CSB-MP518535BRJ
CSB-EP518535BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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