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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Putative carbonic anhydrase-like protein YbcF (ybcF) CSB-YP518536BRJ
CSB-EP518536BRJ
CSB-BP518536BRJ
CSB-MP518536BRJ
CSB-EP518536BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ykoS (ykoS), partial CSB-YP518537BRJ
CSB-EP518537BRJ
CSB-BP518537BRJ
CSB-MP518537BRJ
CSB-EP518537BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yopW (yopW) CSB-YP518538BRJ
CSB-EP518538BRJ
CSB-BP518538BRJ
CSB-MP518538BRJ
CSB-EP518538BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yoeB (yoeB) CSB-YP518539BRJ
CSB-EP518539BRJ
CSB-BP518539BRJ
CSB-MP518539BRJ
CSB-EP518539BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ytmO (ytmO) CSB-YP518540BRJ
CSB-EP518540BRJ
CSB-BP518540BRJ
CSB-MP518540BRJ
CSB-EP518540BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yocN (yocN) CSB-YP518541BRJ
CSB-EP518541BRJ
CSB-BP518541BRJ
CSB-MP518541BRJ
CSB-EP518541BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative sugar kinase YoaC (yoaC) CSB-YP518542BRJ
CSB-EP518542BRJ
CSB-BP518542BRJ
CSB-MP518542BRJ
CSB-EP518542BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yopV (yopV) CSB-YP518543BRJ
CSB-EP518543BRJ
CSB-BP518543BRJ
CSB-MP518543BRJ
CSB-EP518543BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative cysteine desulfurase IscS 2 (iscS2) CSB-YP518544BRJ
CSB-EP518544BRJ
CSB-BP518544BRJ
CSB-MP518544BRJ
CSB-EP518544BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ylbD (ylbD) CSB-YP518545BRJ
CSB-EP518545BRJ
CSB-BP518545BRJ
CSB-MP518545BRJ
CSB-EP518545BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized UDP-glucose epimerase ytcB (ytcB) CSB-YP518546BRJ
CSB-EP518546BRJ
CSB-BP518546BRJ
CSB-MP518546BRJ
CSB-EP518546BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yobS (yobS) CSB-YP518547BRJ
CSB-EP518547BRJ
CSB-BP518547BRJ
CSB-MP518547BRJ
CSB-EP518547BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MscS family protein YkuT (ykuT), partial CSB-YP518548BRJ1
CSB-EP518548BRJ1
CSB-BP518548BRJ1
CSB-MP518548BRJ1
CSB-EP518548BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cob (I)yrinic acid a,c-diamide adenosyltransferase CSB-YP518549BRJ
CSB-EP518549BRJ
CSB-BP518549BRJ
CSB-MP518549BRJ
CSB-EP518549BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ykoX (ykoX), partial CSB-YP518550BRJ1
CSB-EP518550BRJ1
CSB-BP518550BRJ1
CSB-MP518550BRJ1
CSB-EP518550BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0361 protein yobE (yobE) CSB-YP518551BRJ
CSB-EP518551BRJ
CSB-BP518551BRJ
CSB-MP518551BRJ
CSB-EP518551BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yobV (yobV) CSB-YP518552BRJ
CSB-EP518552BRJ
CSB-BP518552BRJ
CSB-MP518552BRJ
CSB-EP518552BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative cytochrome P450 CypX (cypX) CSB-YP518553BRJ
CSB-EP518553BRJ
CSB-BP518553BRJ
CSB-MP518553BRJ
CSB-EP518553BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Dephospho-CoA kinase (coaE) CSB-YP518554BRJ
CSB-EP518554BRJ
CSB-BP518554BRJ
CSB-MP518554BRJ
CSB-EP518554BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ATP-dependent DNA helicase recG (recG), partial CSB-YP518555BRJ
CSB-EP518555BRJ
CSB-BP518555BRJ
CSB-MP518555BRJ
CSB-EP518555BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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