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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Uncharacterized N-acetyltransferase YoaA (yoaA) CSB-YP518496BRJ
CSB-EP518496BRJ
CSB-BP518496BRJ
CSB-MP518496BRJ
CSB-EP518496BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative mannosyltransferase ykcB (ykcB), partial CSB-YP518497BRJ
CSB-EP518497BRJ
CSB-BP518497BRJ
CSB-MP518497BRJ
CSB-EP518497BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived putative HNH endonuclease yoqL (yoqL) CSB-YP518498BRJ
CSB-EP518498BRJ
CSB-BP518498BRJ
CSB-MP518498BRJ
CSB-EP518498BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable flavodoxin-2 (ykuP) CSB-YP518499BRJ
CSB-BP518499BRJ
CSB-MP518499BRJ
CSB-EP518499BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yfkL (yfkL), partial CSB-YP518500BRJ
CSB-EP518500BRJ
CSB-BP518500BRJ
CSB-MP518500BRJ
CSB-EP518500BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yvkN (yvkN) CSB-YP518501BRJ
CSB-EP518501BRJ
CSB-BP518501BRJ
CSB-MP518501BRJ
CSB-EP518501BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable zinc transport system permease protein AdcB (adcB), partial CSB-YP518502BRJ1
CSB-EP518502BRJ1
CSB-BP518502BRJ1
CSB-MP518502BRJ1
CSB-EP518502BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP518503BRJ
CSB-EP518503BRJ
CSB-BP518503BRJ
CSB-MP518503BRJ
CSB-EP518503BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis DNA polymerase III subunit alpha (dnaE), partial CSB-YP518504BRJ
CSB-EP518504BRJ
CSB-BP518504BRJ
CSB-MP518504BRJ
CSB-EP518504BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized lipoprotein yerH (yerH) CSB-YP518505BRJ
CSB-EP518505BRJ
CSB-BP518505BRJ
CSB-MP518505BRJ
CSB-EP518505BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable L-serine dehydratase, beta chain (sdaAB) CSB-YP518506BRJ
CSB-EP518506BRJ
CSB-BP518506BRJ
CSB-MP518506BRJ
CSB-EP518506BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein YerI (yerI) CSB-YP518507BRJ
CSB-EP518507BRJ
CSB-BP518507BRJ
CSB-MP518507BRJ
CSB-EP518507BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yobD (yobD) CSB-YP518508BRJ
CSB-EP518508BRJ
CSB-BP518508BRJ
CSB-MP518508BRJ
CSB-EP518508BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yodI (yodI), partial CSB-YP518509BRJ1
CSB-EP518509BRJ1
CSB-BP518509BRJ1
CSB-MP518509BRJ1
CSB-EP518509BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable 2-dehydropantoate 2-reductase (panE) CSB-YP518510BRJ
CSB-EP518510BRJ
CSB-BP518510BRJ
CSB-MP518510BRJ
CSB-EP518510BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis S-ribosylhomocysteine lyase (luxS) CSB-YP518511BRJ
CSB-BP518511BRJ
CSB-MP518511BRJ
CSB-EP518511BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable cell division protein ytgP (ytgP), partial CSB-YP518512BRJ
CSB-EP518512BRJ
CSB-BP518512BRJ
CSB-MP518512BRJ
CSB-EP518512BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable BsuMI modification methylase subunit ydiP (ydiP) CSB-YP518513BRJ
CSB-EP518513BRJ
CSB-BP518513BRJ
CSB-MP518513BRJ
CSB-EP518513BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator YofA (yofA) CSB-YP518514BRJ
CSB-EP518514BRJ
CSB-BP518514BRJ
CSB-MP518514BRJ
CSB-EP518514BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative niacin/nicotinamide transporter NaiP (naiP), partial CSB-YP518515BRJ
CSB-EP518515BRJ
CSB-BP518515BRJ
CSB-MP518515BRJ
CSB-EP518515BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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