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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptomyces griseus subsp. griseus Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP540912FOF
CSB-EP540912FOF
CSB-BP540912FOF
CSB-MP540912FOF
CSB-EP540912FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L31 type B (rpmE2) CSB-YP540913FOF
CSB-EP540913FOF
CSB-BP540913FOF
CSB-MP540913FOF
CSB-EP540913FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP540914FOF
CSB-EP540914FOF
CSB-BP540914FOF
CSB-MP540914FOF
CSB-EP540914FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Urease accessory protein UreD 2 (ureD2) CSB-YP540915FOF
CSB-EP540915FOF
CSB-BP540915FOF
CSB-MP540915FOF
CSB-EP540915FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP540916FOF
CSB-EP540916FOF
CSB-BP540916FOF
CSB-MP540916FOF
CSB-EP540916FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L21 (rplU) CSB-YP540917FOF
CSB-EP540917FOF
CSB-BP540917FOF
CSB-MP540917FOF
CSB-EP540917FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB, partial CSB-YP540918FOF
CSB-EP540918FOF
CSB-BP540918FOF
CSB-MP540918FOF
CSB-EP540918FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Elongation factor 4 (lepA), partial CSB-YP540919FOF
CSB-EP540919FOF
CSB-BP540919FOF
CSB-MP540919FOF
CSB-EP540919FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus DNA repair protein recO (recO) CSB-YP540920FOF
CSB-EP540920FOF
CSB-BP540920FOF
CSB-MP540920FOF
CSB-EP540920FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 30S ribosomal protein S2 (rpsB) CSB-YP540921FOF
CSB-EP540921FOF
CSB-BP540921FOF
CSB-MP540921FOF
CSB-EP540921FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP540922FOF
CSB-EP540922FOF
CSB-BP540922FOF
CSB-MP540922FOF
CSB-EP540922FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Octanoyltransferase (lipB) CSB-YP540923FOF
CSB-EP540923FOF
CSB-BP540923FOF
CSB-MP540923FOF
CSB-EP540923FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Thiazole synthase (thiG) CSB-YP540924FOF
CSB-EP540924FOF
CSB-BP540924FOF
CSB-MP540924FOF
CSB-EP540924FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus ATP synthase epsilon chain (atpC) CSB-YP540925FOF
CSB-EP540925FOF
CSB-BP540925FOF
CSB-MP540925FOF
CSB-EP540925FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Probable DNA repair protein SGR_2195 (SGR_2195) CSB-YP540926FOF
CSB-EP540926FOF
CSB-BP540926FOF
CSB-MP540926FOF
CSB-EP540926FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Indole-3-glycerol phosphate synthase (trpC) CSB-YP540927FOF
CSB-EP540927FOF
CSB-BP540927FOF
CSB-MP540927FOF
CSB-EP540927FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus UPF0042 nucleotide-binding protein SGR_5570 (SGR_5570) CSB-YP540928FOF
CSB-EP540928FOF
CSB-BP540928FOF
CSB-MP540928FOF
CSB-EP540928FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Tyrosine--tRNA ligase (tyrS) CSB-YP540929FOF
CSB-EP540929FOF
CSB-BP540929FOF
CSB-MP540929FOF
CSB-EP540929FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Arginine repressor (argR) CSB-YP540930FOF
CSB-EP540930FOF
CSB-BP540930FOF
CSB-MP540930FOF
CSB-EP540930FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Threonine--tRNA ligase (thrS), partial CSB-YP540931FOF
CSB-EP540931FOF
CSB-BP540931FOF
CSB-MP540931FOF
CSB-EP540931FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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