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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptomyces griseus subsp. griseus Histidine--tRNA ligase (hisS) CSB-YP540932FOF
CSB-EP540932FOF
CSB-BP540932FOF
CSB-MP540932FOF
CSB-EP540932FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 10 kDa chaperonin (groS) CSB-YP540933FOF
CSB-EP540933FOF
CSB-BP540933FOF
CSB-MP540933FOF
CSB-EP540933FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 30S ribosomal protein S13 (rpsM) CSB-YP540934FOF
CSB-EP540934FOF
CSB-BP540934FOF
CSB-MP540934FOF
CSB-EP540934FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L18 (rplR) CSB-YP540935FOF
CSB-EP540935FOF
CSB-BP540935FOF
CSB-MP540935FOF
CSB-EP540935FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 30S ribosomal protein S17 (rpsQ) CSB-YP540936FOF
CSB-EP540936FOF
CSB-BP540936FOF
CSB-MP540936FOF
CSB-EP540936FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 30S ribosomal protein S19 (rpsS) CSB-YP540937FOF
CSB-EP540937FOF
CSB-BP540937FOF
CSB-MP540937FOF
CSB-EP540937FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L7/L12 (rplL) CSB-YP540938FOF
CSB-EP540938FOF
CSB-BP540938FOF
CSB-MP540938FOF
CSB-EP540938FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus N utilization substance protein B homolog (nusB) CSB-YP540939FOF
CSB-EP540939FOF
CSB-BP540939FOF
CSB-MP540939FOF
CSB-EP540939FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus S-adenosylmethionine synthase (metK) CSB-YP540940FOF
CSB-EP540940FOF
CSB-BP540940FOF
CSB-MP540940FOF
CSB-EP540940FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Undecaprenyl-diphosphatase (uppP), partial CSB-YP540941FOF1
CSB-EP540941FOF1
CSB-BP540941FOF1
CSB-MP540941FOF1
CSB-EP540941FOF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L33 2 (rpmG2) CSB-YP540942FOF
CSB-EP540942FOF
CSB-BP540942FOF
CSB-MP540942FOF
CSB-EP540942FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus NADH-quinone oxidoreductase subunit B 2 (nuoB2) CSB-YP540943FOF
CSB-EP540943FOF
CSB-BP540943FOF
CSB-MP540943FOF
CSB-EP540943FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Urease accessory protein UreG 1 (ureG1) CSB-YP540944FOF
CSB-EP540944FOF
CSB-BP540944FOF
CSB-MP540944FOF
CSB-EP540944FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Ribosomal protein L11 methyltransferase (prmA) CSB-YP540945DZY
CSB-EP540945DZY
CSB-BP540945DZY
CSB-MP540945DZY
CSB-EP540945DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP540946DZY
CSB-EP540946DZY
CSB-BP540946DZY
CSB-MP540946DZY
CSB-EP540946DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP540947DZY
CSB-EP540947DZY
CSB-BP540947DZY
CSB-MP540947DZY
CSB-EP540947DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. UDP-N-acetylmuramate--L-alanine ligase (murC), partial CSB-YP540948DZY
CSB-EP540948DZY
CSB-BP540948DZY
CSB-MP540948DZY
CSB-EP540948DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L3 (rplC) CSB-YP540949DZY
CSB-EP540949DZY
CSB-BP540949DZY
CSB-MP540949DZY
CSB-EP540949DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L16 (rplP) CSB-YP540950DZY
CSB-EP540950DZY
CSB-BP540950DZY
CSB-MP540950DZY
CSB-EP540950DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 30S ribosomal protein S7 (rpsG) CSB-YP540951DZY
CSB-EP540951DZY
CSB-BP540951DZY
CSB-MP540951DZY
CSB-EP540951DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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