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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shigella flexneri serotype 5b 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP605495SGG
CSB-EP605495SGG
CSB-BP605495SGG
CSB-MP605495SGG
CSB-EP605495SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Protein sprT (sprT) CSB-YP605496SGG
CSB-EP605496SGG
CSB-BP605496SGG
CSB-MP605496SGG
CSB-EP605496SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b L-fucose mutarotase (fucU) CSB-YP605497SGG
CSB-EP605497SGG
CSB-BP605497SGG
CSB-MP605497SGG
CSB-EP605497SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Protein nrdI (nrdI) CSB-YP605498SGG
CSB-EP605498SGG
CSB-BP605498SGG
CSB-MP605498SGG
CSB-EP605498SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Anaerobic nitric oxide reductase transcription regulator norR (norR), partial CSB-YP605499SGG
CSB-EP605499SGG
CSB-BP605499SGG
CSB-MP605499SGG
CSB-EP605499SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b DNA mismatch repair protein MutS (mutS), partial CSB-YP605500SGG
CSB-EP605500SGG
CSB-BP605500SGG
CSB-MP605500SGG
CSB-EP605500SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP605501SGG
CSB-EP605501SGG
CSB-BP605501SGG
CSB-MP605501SGG
CSB-EP605501SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP605502SGG
CSB-EP605502SGG
CSB-BP605502SGG
CSB-MP605502SGG
CSB-EP605502SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP605503SGG
CSB-EP605503SGG
CSB-BP605503SGG
CSB-MP605503SGG
CSB-EP605503SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Elongation factor 4 (lepA), partial CSB-YP605504SGG
CSB-EP605504SGG
CSB-BP605504SGG
CSB-MP605504SGG
CSB-EP605504SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD (+) reductase component (hcaD) CSB-YP605505SGG
CSB-EP605505SGG
CSB-BP605505SGG
CSB-MP605505SGG
CSB-EP605505SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b HTH-type transcriptional regulator iscR (iscR) CSB-YP605506SGG
CSB-EP605506SGG
CSB-BP605506SGG
CSB-MP605506SGG
CSB-EP605506SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Cytoskeleton protein rodZ (rodZ), partial CSB-YP605507SGG
CSB-EP605507SGG
CSB-BP605507SGG
CSB-MP605507SGG
CSB-EP605507SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP605508SGG
CSB-EP605508SGG
CSB-BP605508SGG
CSB-MP605508SGG
CSB-EP605508SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Acetyltransferase YpeA (ypeA) CSB-YP605509SGG
CSB-EP605509SGG
CSB-BP605509SGG
CSB-MP605509SGG
CSB-EP605509SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b 3-ketoacyl-CoA thiolase (fadI) CSB-YP605510SGG
CSB-EP605510SGG
CSB-BP605510SGG
CSB-MP605510SGG
CSB-EP605510SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b UPF0304 protein yfbU (yfbU) CSB-YP605511SGG
CSB-EP605511SGG
CSB-BP605511SGG
CSB-MP605511SGG
CSB-EP605511SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP605512SGG
CSB-EP605512SGG
CSB-BP605512SGG
CSB-MP605512SGG
CSB-EP605512SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF), partial CSB-YP605513SGG1
CSB-EP605513SGG1
CSB-BP605513SGG1
CSB-MP605513SGG1
CSB-EP605513SGG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri serotype 5b Bifunctional polymyxin resistance protein ArnA (arnA), partial CSB-YP605514SGG
CSB-EP605514SGG
CSB-BP605514SGG
CSB-MP605514SGG
CSB-EP605514SGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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