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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodopseudomonas palustris Molybdenum import ATP-binding protein ModC (modC) CSB-YP617847RAAG
CSB-EP617847RAAG
CSB-BP617847RAAG
CSB-MP617847RAAG
CSB-EP617847RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris ATP synthase subunit a (atpB), partial CSB-YP617848RAAG1
CSB-EP617848RAAG1
CSB-BP617848RAAG1
CSB-MP617848RAAG1
CSB-EP617848RAAG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Protoheme IX farnesyltransferase (ctaB), partial CSB-YP617849RAAG
CSB-EP617849RAAG
CSB-BP617849RAAG
CSB-MP617849RAAG
CSB-EP617849RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP617850RAAG
CSB-EP617850RAAG
CSB-BP617850RAAG
CSB-MP617850RAAG
CSB-EP617850RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris GTPase obg (obg) CSB-YP617851RAAG
CSB-EP617851RAAG
CSB-BP617851RAAG
CSB-MP617851RAAG
CSB-EP617851RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris ATP synthase subunit alpha (atpA), partial CSB-YP617852RAAG
CSB-EP617852RAAG
CSB-BP617852RAAG
CSB-MP617852RAAG
CSB-EP617852RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Cytochrome c biogenesis ATP-binding export protein CcmA (ccmA) CSB-YP617853RAAG
CSB-EP617853RAAG
CSB-BP617853RAAG
CSB-MP617853RAAG
CSB-EP617853RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Leucine--tRNA ligase (leuS), partial CSB-YP617854RAAG
CSB-EP617854RAAG
CSB-BP617854RAAG
CSB-MP617854RAAG
CSB-EP617854RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Tryptophan synthase beta chain (trpB) CSB-YP617855RAAG
CSB-EP617855RAAG
CSB-BP617855RAAG
CSB-MP617855RAAG
CSB-EP617855RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP617856RAAG
CSB-EP617856RAAG
CSB-BP617856RAAG
CSB-MP617856RAAG
CSB-EP617856RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP617857RAAG
CSB-EP617857RAAG
CSB-BP617857RAAG
CSB-MP617857RAAG
CSB-EP617857RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Peptide deformylase (def) CSB-YP617858RAAG
CSB-EP617858RAAG
CSB-BP617858RAAG
CSB-MP617858RAAG
CSB-EP617858RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Putative ribose/galactose/methyl galactoside import ATP-binding protein (Bxeno_C1272), partial CSB-YP617859BNV
CSB-EP617859BNV
CSB-BP617859BNV
CSB-MP617859BNV
CSB-EP617859BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 60 kDa chaperonin 5 (groL5), partial CSB-YP617860BNV
CSB-EP617860BNV
CSB-BP617860BNV
CSB-MP617860BNV
CSB-EP617860BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Phosphonates import ATP-binding protein PhnC (phnC) CSB-YP617861BNV
CSB-EP617861BNV
CSB-BP617861BNV
CSB-MP617861BNV
CSB-EP617861BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans L-rhamnose mutarotase (rhaM) CSB-YP617862BNV
CSB-EP617862BNV
CSB-BP617862BNV
CSB-MP617862BNV
CSB-EP617862BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Betaine aldehyde dehydrogenase (betB) CSB-YP617863BNV
CSB-EP617863BNV
CSB-BP617863BNV
CSB-MP617863BNV
CSB-EP617863BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans D- (-)-3-hydroxybutyrate oligomer hydrolase (Bxeno_B0795), partial CSB-YP617864BNV
CSB-EP617864BNV
CSB-BP617864BNV
CSB-MP617864BNV
CSB-EP617864BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 4-hydroxy-2-oxovalerate aldolase 3 (Bxeno_B0695) CSB-YP617865BNV
CSB-EP617865BNV
CSB-BP617865BNV
CSB-MP617865BNV
CSB-EP617865BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Malate dehydrogenase (mdh) CSB-YP617866BNV
CSB-EP617866BNV
CSB-BP617866BNV
CSB-MP617866BNV
CSB-EP617866BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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