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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia xenovorans Pyridoxamine kinase (pdxY) CSB-YP617907BNV
CSB-EP617907BNV
CSB-BP617907BNV
CSB-MP617907BNV
CSB-EP617907BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Early lymphoid activation gene protein (EPAG) CSB-YP617908HU
CSB-EP617908HU
CSB-BP617908HU
CSB-MP617908HU
CSB-EP617908HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human E3 ubiquitin/ISG15 ligase TRIM25 (TRIM25) CSB-YP617909HU
CSB-EP617909HU
CSB-BP617909HU
CSB-MP617909HU
CSB-EP617909HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP617910BNV1
CSB-EP617910BNV1
CSB-BP617910BNV1
CSB-MP617910BNV1
CSB-EP617910BNV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP617911BNV
CSB-EP617911BNV
CSB-BP617911BNV
CSB-MP617911BNV
CSB-EP617911BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Probable transcriptional regulatory protein Bxeno_A1185 (Bxeno_A1185) CSB-YP617912BNV
CSB-EP617912BNV
CSB-BP617912BNV
CSB-MP617912BNV
CSB-EP617912BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Peptidyl-prolyl cis-trans isomerase FKBP8 (FKBP8), partial CSB-YP617913HU1
CSB-EP617913HU1
CSB-BP617913HU1
CSB-MP617913HU1
CSB-EP617913HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Deoxycytidine triphosphate deaminase (dcd) CSB-YP617914BNV
CSB-EP617914BNV
CSB-BP617914BNV
CSB-MP617914BNV
CSB-EP617914BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP617915BNV1
CSB-EP617915BNV1
CSB-BP617915BNV1
CSB-MP617915BNV1
CSB-EP617915BNV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Phosphatidylinositol N-acetylglucosaminyltransferase subunit H (PIGH) CSB-YP617916HU
CSB-EP617916HU
CSB-BP617916HU
CSB-MP617916HU
CSB-EP617916HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Beclin-1 (BECN1) CSB-YP617917HU
CSB-BP617917HU
CSB-MP617917HU
CSB-EP617917HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Putative Holliday junction resolvase (Bxeno_A0650) CSB-YP617918BNV
CSB-EP617918BNV
CSB-BP617918BNV
CSB-MP617918BNV
CSB-EP617918BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Hyaluronan-binding protein 2 (HABP2), partial CSB-YP617919HU
CSB-EP617919HU
CSB-BP617919HU
CSB-MP617919HU
CSB-EP617919HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Meiotic recombination protein DMC1/LIM15 homolog (DMC1) CSB-YP617920HU
CSB-EP617920HU
CSB-BP617920HU
CSB-MP617920HU
CSB-EP617920HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Transcriptional repressor NrdR (nrdR) CSB-YP617921BNV
CSB-EP617921BNV
CSB-BP617921BNV
CSB-MP617921BNV
CSB-EP617921BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP617922BNV
CSB-EP617922BNV
CSB-BP617922BNV
CSB-MP617922BNV
CSB-EP617922BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human E3 ubiquitin-protein ligase TRIP12 (TRIP12), partial CSB-YP617924HU
CSB-MP617924HU
CSB-EP617924HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tubulin polyglutamylase TTLL4 (TTLL4), partial CSB-YP617925HU
CSB-EP617925HU
CSB-BP617925HU
CSB-MP617925HU
CSB-EP617925HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DNA replication complex GINS protein PSF1 (GINS1) CSB-YP617926HU
CSB-EP617926HU
CSB-BP617926HU
CSB-MP617926HU
CSB-EP617926HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Neutral alpha-glucosidase AB (GANAB), partial CSB-YP617927HU
CSB-EP617927HU
CSB-BP617927HU
CSB-MP617927HU
CSB-EP617927HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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