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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S16 (rpsP) CSB-YP622699RAAG
CSB-EP622699RAAG
CSB-BP622699RAAG
CSB-MP622699RAAG
CSB-EP622699RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP622700RAAG
CSB-EP622700RAAG
CSB-BP622700RAAG
CSB-MP622700RAAG
CSB-EP622700RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP622701RAAG
CSB-EP622701RAAG
CSB-BP622701RAAG
CSB-MP622701RAAG
CSB-EP622701RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP622702RAAG
CSB-EP622702RAAG
CSB-BP622702RAAG
CSB-MP622702RAAG
CSB-EP622702RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP622703RAAG
CSB-EP622703RAAG
CSB-BP622703RAAG
CSB-MP622703RAAG
CSB-EP622703RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Probable rRNA maturation factor (RPD_0240) CSB-YP622704RAAG
CSB-EP622704RAAG
CSB-BP622704RAAG
CSB-MP622704RAAG
CSB-EP622704RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Catalase-peroxidase (katG), partial CSB-YP622705RAAG
CSB-EP622705RAAG
CSB-BP622705RAAG
CSB-MP622705RAAG
CSB-EP622705RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Undecaprenyl-diphosphatase (uppP), partial CSB-YP622706RAAG1
CSB-EP622706RAAG1
CSB-BP622706RAAG1
CSB-MP622706RAAG1
CSB-EP622706RAAG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UPF0133 protein RPD_0086 (RPD_0086) CSB-YP622707RAAG
CSB-EP622707RAAG
CSB-BP622707RAAG
CSB-MP622707RAAG
CSB-EP622707RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Chromosomal replication initiator protein DnaA (dnaA) CSB-YP622708RAAG
CSB-EP622708RAAG
CSB-BP622708RAAG
CSB-MP622708RAAG
CSB-EP622708RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Taurine import ATP-binding protein TauB 3 (tauB3) CSB-YP622709BNV
CSB-EP622709BNV
CSB-BP622709BNV
CSB-MP622709BNV
CSB-EP622709BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans PKHD-type hydroxylase Bxeno_B2194 (Bxeno_B2194) CSB-YP622710BNV
CSB-EP622710BNV
CSB-BP622710BNV
CSB-MP622710BNV
CSB-EP622710BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans L-rhamnonate dehydratase (rhmD) CSB-YP622711BNV
CSB-EP622711BNV
CSB-BP622711BNV
CSB-MP622711BNV
CSB-EP622711BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 1-aminocyclopropane-1-carboxylate deaminase (acdS) CSB-YP622712BNV
CSB-EP622712BNV
CSB-BP622712BNV
CSB-MP622712BNV
CSB-EP622712BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Formimidoylglutamase (hutG) CSB-YP622713BNV
CSB-EP622713BNV
CSB-BP622713BNV
CSB-MP622713BNV
CSB-EP622713BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP622714BNV
CSB-EP622714BNV
CSB-BP622714BNV
CSB-MP622714BNV
CSB-EP622714BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Aliphatic sulfonates import ATP-binding protein SsuB 2 (ssuB2) CSB-YP622715BNV
CSB-EP622715BNV
CSB-BP622715BNV
CSB-MP622715BNV
CSB-EP622715BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP622716BNV
CSB-EP622716BNV
CSB-BP622716BNV
CSB-MP622716BNV
CSB-EP622716BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans tRNA modification GTPase MnmE (mnmE) CSB-YP622717BNV
CSB-EP622717BNV
CSB-BP622717BNV
CSB-MP622717BNV
CSB-EP622717BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP622718BNV
CSB-EP622718BNV
CSB-BP622718BNV
CSB-MP622718BNV
CSB-EP622718BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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